FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences199689
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7070.3540505486030778No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT4200.21032705857608583No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT2970.1487312771359464No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA2880.1442242687378874No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG2810.14071881776161932No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA2590.12970168612191957No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC2520.12619623514565148No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT2520.12619623514565148No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC2460.12319156288027884No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC2410.12068766932580163No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC2370.11868455448221986No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT2120.1061650867098338No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT2100.10516352928804291No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA2080.10416197186625202No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG2060.10316041444446114No Hit
CATCTAAGGAAGGCAGCAGGCGCGTAAATTACCCACTCCCAGCTCGGGGA2000.10015574217908849No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAT150.002222205570.01
ACTGAGC200.006943668252.528
CTAAAGA200.006943668252.54
TCTAGCC400.002414664535.08
ATATAAG400.002414664535.02
TGACTAG400.002414664535.025
CAGAATA552.984666E-431.8181816
AGAATAT552.984666E-431.8181817
GAATAAT450.004300020631.1111131
CTACAGC450.004300020631.1111134
GTGGTTA450.004300020631.11111316
TCTATGC450.004300020631.1111133
GTTAGAG703.4765646E-530.00000262
ATAGGGG604.96973E-429.1666663
ACCAATA1202.2773747E-929.16666639
AACTACA604.96973E-429.1666662
AGATCCG500.0071963328.00000248
ATAGGCC500.0071963328.0000023
GAGTACG500.0071963328.0000021
TAGATCC500.0071963328.00000247
Produced by FastQC (version 0.11.2)