FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176779
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5410.3060318250470927No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT4000.22627121999785044No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA3150.1781885857483072No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG3080.17422883939834483No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA2680.15160171739855977No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT2570.1453792588486189No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT2370.13406569784872638No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC2200.12444917099881773No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC2130.12048942464885536No Hit
CATATAAGAACTCCACCGGTAATACGCTTACATACATAAAGGTATAGTAC1980.11200425389893595No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG1950.11030721974895209No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA1940.10974154169895746No Hit
GTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAA1910.10804450754897357No Hit
CATCTAAGGAAGGCAGCAGGCGCGTAAATTACCCACTCCCAGCTCGGGGA1880.10634747339898971No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT1850.10465043924900583No Hit
GATATGAGTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGAGCTGGGA1780.10069069289904344No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGTA200.006942352352.50000451
TTATACG200.006942352352.5000044
ATTTTTA200.006942352352.50000434
GTGCTAG200.006942352352.5000041
TGGATTC200.006942352352.50000431
CATACGT305.865832E-446.6666642
TACTAGT305.865832E-446.6666642
TAAGGAG305.865832E-446.6666645
ACTAGTT350.001252887540.03
TACGTCT350.001252887540.04
TCTAGGG459.1703216E-538.8888852
TTTCGGA556.6670946E-638.1818161
GTACTAG400.002413914835.0000041
GTTCAGG400.002413914835.0000041
AGTAGTA400.002413914835.00000425
GCAGACC400.002413914835.00000419
ATATAAG601.21074045E-535.02
TATAAGA601.21074045E-535.03
CGCGTAA1154.0017767E-1133.47826421
TAAATTA1055.4387783E-1033.33333225
Produced by FastQC (version 0.11.2)