FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences249831
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8100.32421917216038043No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT6460.25857479656247623No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG5280.21134286777861835No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA4420.17691959764801007No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA4360.17451797415052575No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT3810.152503092090253No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC3770.15090200975859683No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT3730.1493009274269406No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC3430.13729280993951912No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG3370.13489118644203482No Hit
GTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAA3260.13048821002998026No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT3030.12128198662295711No Hit
CATCTAAGGAAGGCAGCAGGCGCGTAAATTACCCACTCCCAGCTCGGGGA2830.11327657496467611No Hit
CATATAAGAACTCCACCGGTAATACGCTTACATACATAAAGGTATAGTAC2690.10767278680387943No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA2630.10527116330639513No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAG2520.10086818689434057No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGAT207.916494E-570.03
CCGCTAG200.006945701352.537
AGTCCCC400.002415822635.041
TAGTGTA1600.030.62570
TCTGTCA604.973265E-429.1666668
TTACGGC604.973265E-429.16666618
ATACCTT853.942705E-628.823536
GTATTGG755.570834E-528.01
ATCTAGT755.570834E-528.07
CTATACC755.570834E-528.04
GACCGTT500.0071997427.9999987
GGACTGG657.941813E-426.9230776
CTAAGCA657.941813E-426.9230774
ACTATAC808.6440414E-526.253
TCTAGGG1101.0115782E-625.4545442
AACGGGT2600.024.23076822
TTGATAG2350.023.82978824
TCTAGGA2950.023.7288152
CAGTACC750.001829036423.3333344
CTAGGAC2700.023.3333343
Produced by FastQC (version 0.11.2)