FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1745
Sequences flagged as poor quality0
Sequence length76
%GC78

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG83347.73638968481375No Hit
CTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTG291.66189111747851No Hit
GCCCTCGGGCTATGCGCTGGGCATCGACACCACCGCCTTTGCCGCCGGTA30.17191977077363896No Hit
TGCCGCCGAACATCACCCACGACAGCACGGCAAAGATATTCTTGTGATCG20.1146131805157593No Hit
ATATTCAGCAGCTTGGACTCCCCCCACAGACACTGTATACCTGTCTCTTA20.1146131805157593No Hit
TACTTATTTTTTGATTGGAGCTTACGGTATACGTCTGCCAATGTTTGTTC20.1146131805157593No Hit
GCTCGAAGCGCGTCACGTGGCCATGGAACGGCCGCAGTGCGCTTCGGCTC20.1146131805157593No Hit
GGTCTGCACCATGAACGGCCCGAAGATGTTGAACACCAGCAGGATGCCTG20.1146131805157593No Hit
GAGGAGGCTGTCGCGGGCGCGAAGCAGTTCACCGCGCTCGGCGTACAACG20.1146131805157593No Hit
GCACGCGCAGGGTTTCGTGATCTGCGTGCCCGACGTGAAGCTCGGACCTG20.1146131805157593No Hit
GTGCCGGAACCAGCAATTCCGCCAAGGCCACCGCCGAGCGATCCGCCAAG20.1146131805157593No Hit
TCCATGCGGCGGACCTGCTTTTCGGCCTGGTCCATGTTGGCACCTTGCGC20.1146131805157593No Hit
TACCTGACCCACGAAAGACTCACCGGGGAAGATATCCGGCCAAGCAATAA20.1146131805157593No Hit
GCCTTGAACTCAGCATCACCGAACCAGTAAGGCTGCACCACCTGTCTCTT20.1146131805157593No Hit
GGACTCGAACCTGCGACCAACCGGTTAACAGCCGGCCGCTCTACCTGCTG20.1146131805157593No Hit
GCCCCTGTCCGATGCACTGCCGCATACGACGGTCATCAGCCGCGCCGATA20.1146131805157593No Hit
AGGCCTAAGACATCTCTGGTAAGATACGATACAGTTATTGAATGAAAAAG20.1146131805157593No Hit
GGCGTGGTGAGCCTGCGCATGCAGGGCGCCTGCGCGGGTTGCCCCTCCTC20.1146131805157593No Hit
GGGCTAGAGCAATCCCATGATGGGTGACCCATTGGGAAGTTGCTCGTGAG20.1146131805157593No Hit
GTGCTCTTCCGATCTAGAGACGTGTGCTCTTCCGATCTAGAGACGTGTGC20.1146131805157593Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTC20.1146131805157593No Hit
CTTCGGCAACGCGGCAATGGCGGCATGGTCCGTCATACAGGCGCAGGCGC20.1146131805157593No Hit
AGGCGGCCATGGCGTCAAAGCGCCAGTAGGCCGGCACCCACTTCGGGTTG20.1146131805157593No Hit
GTCCCCAGCTCGGCGCCGAAGGCCTTCAAGGCTGGGTCATCCGCACCCGC20.1146131805157593No Hit
TCCTTAGATCGTTAACAGTTACTTGGATAACTGTGGTAATTCTAGAGCTA20.1146131805157593No Hit
GGACAGTAATAAGGACAAAGTGATTTTATATGTGCGTGTGTGAGAGCAAG20.1146131805157593No Hit
CACCATAACATGCTGGCAACAGTGAACCAAGGGTTGCGCTCGTTGCGGGA20.1146131805157593No Hit
GGAAATTACCGCCTGGACCGAAGACGGCGAAATCATGGGCGTGCGCCACA20.1146131805157593No Hit
GCTGCAGACAAGAAATGTCGATTCATCATGAACCCCAGGTATCAAAGAAG20.1146131805157593No Hit
CCTCCTTACCGGCTGACCCCTTGGAACATGCCTTTGGACGGGTTGTGGCG20.1146131805157593No Hit
AAAATAACCACTATATGAAAATGTGTTTCTTAACCTCATTTGTGATGAGC20.1146131805157593No Hit
GAAGAGCACACGTCTCTAGATCGGAAGAGCACACGTCTCTAGATCGGAAG20.1146131805157593Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GGCTTGCCCGGGAGGCTGTCGAGGCTGGGGTAGGCCGTCACGCCGGGACG20.1146131805157593No Hit
GTATTGCGCCCACTCGAATGCGGCCGTCTCCGTAAAGTTCACCATGCCCG20.1146131805157593No Hit
CCACGCCGCTGCGTGCGCGGCCGCAGGCCTCTCACGGGCCCTACGGTGCG20.1146131805157593No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)