FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences290671
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGGGCTGCACGCGCGCTACAATGAAAGTATCAACGTGTATTTCCTA22180.7630620185708241No Hit
TTCCTAGACCGAGAGGTCCGGGTAAACCGCTGAACCACTTTCATGCTTGG20140.692879578630135No Hit
GACGTAATCAATGCGAGTTAATGACTCACACTTACTGGGAATTCCAAGTT19960.6866870104000744No Hit
GTCTAGGAAATACACGTTGATACTTTCATTGTAGCGCGCGTGCAGCCCAG18250.6278576122144968No Hit
CACTTACTGGGAATTCCAAGTTCATGTGAACAGTTTCAGTTCACAATCCC15080.5187996050517596No Hit
GACTCACACTTACTGGGAATTCCAAGTTCATGTGAACAGTTTCAGTTCAC13840.4761396905780074No Hit
TTCCAAGTTCATGTGAACAGTTTCAGTTCACAATCCCAAGCATGAAAGTG12950.4455208809960402No Hit
TAATGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCTGGGC11670.4014848402489412No Hit
GTTTCAGTTCACAATCCCAAGCATGAAAGTGGTTCAGCGGTTTACCCGGA10940.3763705357603614No Hit
GGACACTAGCTTCTTAAATGGACAAATTGCGTCTAGCAATAATGAGATTG10840.3729302200769943No Hit
TCACAATCCCAAGCATGAAAGTGGTTCAGCGGTTTACCCGGACCTCTCGG10690.3677697465519436No Hit
GATAAGGACACTAGCTTCTTAAATGGACAAATTGCGTCTAGCAATAATGA10330.35538461009182204No Hit
GCATGAAAGTGGTTCAGCGGTTTACCCGGACCTCTCGGTCTAGGAAATAC9740.3350867475599561No Hit
GCCCAGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCATTA9550.3285501477615586No Hit
GAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCTGGGCTGCACGCGCG9350.3216695163948244No Hit
CCTTAGATGTCCTGGGCTGCACGCGCGCTACAATGAAAGTATCAACGTGT9030.3106605062080497No Hit
CTTACTGGGAATTCCAAGTTCATGTGAACAGTTTCAGTTCACAATCCCAA8980.3089403483663661No Hit
GGTCTGTGATGCCCTTAGATGTCCTGGGCTGCACGCGCGCTACAATGAAA8920.30687615895634585No Hit
GTATTTCCTAGACCGAGAGGTCCGGGTAAACCGCTGAACCACTTTCATGC8780.3020597169996319No Hit
CTTCCGATCTGGGCAGGGACGTAATCAATGCGAGTTAATGACTCACACTT8680.2986194013162648No Hit
CTACAATGAAAGTATCAACGTGTATTTCCTAGACCGAGAGGTCCGGGTAA8660.29793133817959133No Hit
ACAATGAAAGTATCAACGTGTATTTCCTAGACCGAGAGGTCCGGGTAAAC8480.2917387699495306No Hit
GCAATAATGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCT8470.29139473838119384No Hit
TAACAGGTCTGTGATGCCCTTAGATGTCCTGGGCTGCACGCGCGCTACAA8300.2855462017194698No Hit
CCCTTAGATGTCCTGGGCTGCACGCGCGCTACAATGAAAGTATCAACGTG8260.28417007544612294No Hit
GGATAAGGACACTAGCTTCTTAAATGGACAAATTGCGTCTAGCAATAATG8200.28210588603610265No Hit
CATGTGAACAGTTTCAGTTCACAATCCCAAGCATGAAAGTGGTTCAGCGG8070.27763347564772545No Hit
GTATCAACGTGTATTTCCTAGACCGAGAGGTCCGGGTAAACCGCTGAACC7840.2697207495759811No Hit
ATCCCAAGCATGAAAGTGGTTCAGCGGTTTACCCGGACCTCTCGGTCTAG7820.26903268643930767No Hit
GGGATAAGGACACTAGCTTCTTAAATGGACAAATTGCGTCTAGCAATAAT7590.2611199603675633No Hit
TCATTGTAGCGCGCGTGCAGCCCAGGACATCTAAGGGCATCACAGACCTG6970.23979000313068727No Hit
TAGATGTCCTGGGCTGCACGCGCGCTACAATGAAAGTATCAACGTGTATT6960.23944597156235056No Hit
GTTATTGCTCAATCTCATTATTGCTAGACGCAATTTGTCCATTTAAGAAG6430.2212122984405049No Hit
CTTTCATGCTTGGGATTGTGAACTGAAACTGTTCACATGAACTTGGAATT6410.22052423530383147No Hit
CTTTCATTGTAGCGCGCGTGCAGCCCAGGACATCTAAGGGCATCACAGAC6350.2184600458938112No Hit
AGGAAATACACGTTGATACTTTCATTGTAGCGCGCGTGCAGCCCAGGACA6280.21605182491545422No Hit
CAATGAAAGTATCAACGTGTATTTCCTAGACCGAGAGGTCCGGGTAAACC6260.21536376177878083No Hit
AGCAATAACAGGTCTGTGATGCCCTTAGATGTCCTGGGCTGCACGCGCGC6080.20917119354872002No Hit
GTGTATTTCCTAGACCGAGAGGTCCGGGTAAACCGCTGAACCACTTTCAT5850.2012584674769757No Hit
GGGCAGGGACGTAATCAATGCGAGTTAATGACTCACACTTACTGGGAATT5740.1974741202252719No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC5730.19713008865693515No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5510.18956139415352752No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA5430.18680914160683385No Hit
CGTTGATACTTTCATTGTAGCGCGCGTGCAGCCCAGGACATCTAAGGGCA5360.18440092062847688No Hit
GTTCAGCGGTTTACCCGGACCTCTCGGTCTAGGAAATACACGTTGATACT5290.1819926996501199No Hit
TAATCAATGCGAGTTAATGACTCACACTTACTGGGAATTCCAAGTTCATG5120.17614416298839583No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCATTATTGCTAGA5080.17476803671504895No Hit
GTGATGCCCTTAGATGTCCTGGGCTGCACGCGCGCTACAATGAAAGTATC5040.17339191044170213No Hit
GCCCTTAGATGTCCTGGGCTGCACGCGCGCTACAATGAAAGTATCAACGT5030.17304787887336542No Hit
TCACAGACCTGTTATTGCTCAATCTCATTATTGCTAGACGCAATTTGTCC4710.16203886868659068No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC4670.16066274241324383No Hit
GACAAATTGCGTCTAGCAATAATGAGATTGAGCAATAACAGGTCTGTGAT4640.1596306477082337No Hit
AGCTTCTTAAATGGACAAATTGCGTCTAGCAATAATGAGATTGAGCAATA4620.15894258457156027No Hit
GTTAATGACTCACACTTACTGGGAATTCCAAGTTCATGTGAACAGTTTCA4470.15378211104650963No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT4320.14862163752145896No Hit
TCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCATTATTGCTAGACG4320.14862163752145896No Hit
GAAAGTATCAACGTGTATTTCCTAGACCGAGAGGTCCGGGTAAACCGCTG4280.14724551124811214No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT4240.1458693849747653No Hit
ATAACAGGTCTGTGATGCCCTTAGATGTCCTGGGCTGCACGCGCGCTACA4130.14208503772306147No Hit
ACCGAGAGGTCCGGGTAAACCGCTGAACCACTTTCATGCTTGGGATTGTG4060.1396768167447045No Hit
GTACAAAGGGCAGGGACGTAATCAATGCGAGTTAATGACTCACACTTACT4050.13933278517636777No Hit
AGTTAATGACTCACACTTACTGGGAATTCCAAGTTCATGTGAACAGTTTC4000.1376126273346842No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAG3910.13451634321965383No Hit
TCCTAGACCGAGAGGTCCGGGTAAACCGCTGAACCACTTTCATGCTTGGG3880.13348424851464372No Hit
AGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCTGGGCTGCAC3860.13279618537797028No Hit
GGGTAAACCGCTGAACCACTTTCATGCTTGGGATTGTGAACTGAAACTGT3820.13142005910462345No Hit
TTTCATTGTAGCGCGCGTGCAGCCCAGGACATCTAAGGGCATCACAGACC3820.13142005910462345No Hit
CTCACACTTACTGGGAATTCCAAGTTCATGTGAACAGTTTCAGTTCACAA3810.13107602753628672No Hit
GGTTTACCCGGACCTCTCGGTCTAGGAAATACACGTTGATACTTTCATTG3800.13073199596795004No Hit
CTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAAT3800.13073199596795004No Hit
CCTCTCGGTCTAGGAAATACACGTTGATACTTTCATTGTAGCGCGCGTGC3770.1296999012629399No Hit
GAAATACACGTTGATACTTTCATTGTAGCGCGCGTGCAGCCCAGGACATC3720.12797974342125634No Hit
ATCTCATTATTGCTAGACGCAATTTGTCCATTTAAGAAGCTAGTGTCCTT3580.1231633014645424No Hit
GTTCACAATCCCAAGCATGAAAGTGGTTCAGCGGTTTACCCGGACCTCTC3450.11869089107616514No Hit
GAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGA3450.11869089107616514No Hit
CACACTTACTGGGAATTCCAAGTTCATGTGAACAGTTTCAGTTCACAATC3330.11456251225612463No Hit
ACCCGGACCTCTCGGTCTAGGAAATACACGTTGATACTTTCATTGTAGCG3310.1138744491194512No Hit
GCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATGAA3300.11353041755111448No Hit
GTTCATGTGAACAGTTTCAGTTCACAATCCCAAGCATGAAAGTGGTTCAG3300.11353041755111448No Hit
CCGCTGAACCACTTTCATGCTTGGGATTGTGAACTGAAACTGTTCACATG3240.11146622814109423No Hit
GCTTGGGATTGTGAACTGAAACTGTTCACATGAACTTGGAATTCCCAGTA3140.10802591245772711No Hit
GAACAGTTTCAGTTCACAATCCCAAGCATGAAAGTGGTTCAGCGGTTTAC3130.10768188088939042No Hit
CTTCTTAAATGGACAAATTGCGTCTAGCAATAATGAGATTGAGCAATAAC3120.1073378493210537No Hit
GTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGATTAATGAAAAC3110.10699381775271699No Hit
GATCTGGGCAGGGACGTAATCAATGCGAGTTAATGACTCACACTTACTGG3090.10630575461604358No Hit
GATTAATAGAAGCAGTTTGGGGGCATTAGTATTACGACGCGAGAGGTGAA3060.10527365991103343No Hit
AACTGAAACTGTTCACATGAACTTGGAATTCCCAGTAAGTGTGAGTCATT3060.10527365991103343No Hit
GAGTTAATGACTCACACTTACTGGGAATTCCAAGTTCATGTGAACAGTTT3040.10458559677436001No Hit
GGATTGTGAACTGAAACTGTTCACATGAACTTGGAATTCCCAGTAAGTGT2980.10252140736433976No Hit
GTTCACATGAACTTGGAATTCCCAGTAAGTGTGAGTCATTAACTCGCATT2940.10114528109099293No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCCTC252.8356517E-670.0000147
TTAGTCG150.002223227970.070
ATTTTGT200.006946838552.518
ATCTTTT200.006946838552.57
ATAAGGT200.006946838552.54
AGTCGTG200.006946838552.539
TCTGTCC750.051.3333328
ATCTGTG850.049.4117667
TATTCCG305.872117E-446.6666645
CTGTGCT305.872117E-446.6666649
GCGGGGT404.586296E-543.7514
CGATCTG12900.043.1395345
CTGTCCT659.980795E-943.0769239
TTCCGAT23450.042.686572
TCCGATC23550.042.6539233
CTTCCGA23800.042.3529431
CCGATCT24000.042.04
ATCTGGG5350.041.8691567
TCTGGGC3800.041.4473728
CGGGTTT350.001254221740.015
Produced by FastQC (version 0.11.2)