H3W35AFXY_n02_adr3wtzt14s23.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H3W35AFXY_n02_adr3wtzt14s23.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	93734
Sequences flagged as poor quality	0
Sequence length	76
%GC	53

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	261	0.2784475217103719	No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC	236	0.25177630315573857	No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC	203	0.21657029466362257	No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC	190	0.20270126101521327	No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC	161	0.17176264749183862	No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA	148	0.1578936138434293	Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC	138	0.14722512642157595	Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGTAAG	15	0.0022226113	69.967445	1
TAAGCCT	25	2.385156E-4	55.973965	4
TGCATCT	20	0.0069303326	52.503605	10
AGGTTTC	20	0.0069303326	52.503605	32
GCCTGTG	35	0.001249451	40.002743	7
ATCTTTT	40	0.0024073315	35.002403	7
TCTCAGC	40	0.0024073315	35.002403	8
CCTGTGA	45	0.0042870366	31.113247	8
AACATGA	45	0.0042870366	31.113247	56
TACTAAG	45	0.0042982604	31.096647	2
GTCTTGG	45	0.0042982604	31.096647	1
CTAGGAC	45	0.0042982604	31.096647	3
CGGTGTA	95	2.776378E-7	29.475708	70
ATCTATG	50	0.0071747224	28.001925	7
AAACGGC	50	0.0071747224	28.001925	69
GAAACGG	50	0.0071747224	28.001925	68
ACATGAA	50	0.0071747224	28.001925	57
CTCAGAT	50	0.0071747224	28.001925	26
ACGGTGT	150	2.7284841E-11	28.00192	70
ATCCGAT	50	0.0071934606	27.986982	2