FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224499
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6300.28062485801718495No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC3290.1465485369645299No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA2730.12160410514078014No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT2720.12115866885821318No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT2590.1153679971848427No Hit
GAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGA2400.10690470781607045No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT2250.10022316357756605No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTACC200.006939542552.51002526
CGAGTGG200.006945661352.4983346
ATAAGCG200.006945661352.498333
TATAGGT200.006945661352.498334
ATTCGTA305.870161E-446.6651837
ATAGGTA350.001253807539.9987265
ACATAAC400.00241567934.9988863
CAATATA601.2124619E-534.99888641
AGGTGTA2950.032.04001670
GTACCAG450.004301819531.110121
TGTTGTG450.004301819531.110125
GATCTGG4300.028.4874656
ATTAGGG500.007199323327.9991112
GGTTGGT500.007199323327.99911164
TAAGGTG500.007199323327.9991115
TACACCG500.007199323327.9991115
AATATAA755.569558E-527.99910742
TAGGTGT3450.027.39653669
CATGTTA657.9304323E-426.92821921
CCAATAT657.9407654E-426.92221840