FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences335990
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC14300.4256079049971725No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC12050.35864162623887613No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC11630.34614125420399416No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC11560.3440578588648472No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT6890.20506562695318314No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6610.19673204559659516No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA5970.17768385963867972Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC5540.1648858596982053Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG5350.15923092949194917No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA4970.1479210690794369No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT4670.13899223191166404No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA4470.13303967379981546No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC4280.12738474359355934No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT4150.12351558082085777No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC3920.1166701389922319No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG3910.11637251108663949No Hit
GAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTC3600.1071460460132742No Hit
GTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAA3580.10655079020208935No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT3450.10268162742938779No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTGCA350.001255471639.9937067
CACGTTG501.707998E-434.99970213
TTCGGAA652.0976018E-532.3122252
TAGCGCG552.9917396E-431.81317532
TAGGGGT450.00430120731.115484
ATACTTT703.480231E-530.00421120
TGATACT604.9727864E-429.17076118
GGTCCAA1202.2901077E-929.16641811
ACGTGAT1202.2901077E-929.16641869
GCGTGCA604.9771235E-429.16641838
TACACGT604.9771235E-429.16641811
TTAGCAC500.00719828628.0039313
GATTATG500.00719828628.0039312
AAACTTT500.007203520327.99976226
ACGCATC500.007203520327.99976244
AAGACAG500.007203520327.9997625
TGCATAG500.007203520327.99976229
GTGAGCG500.007203520327.9997625
CGGTCCA1253.5415724E-927.99976210
GGGCTGC755.5824556E-527.9955966
Produced by FastQC (version 0.11.2)