FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239550
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9120.3807138384470883No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATAG150.002222749470.05
ATTCTAC200.00694535552.51
CATGTTA350.0012537840.00000421
ATCTGGT1350.036.29637
GATCTGG4550.036.1538436
ATCTAGC400.002415625135.07
ATCTAAC400.002415625135.07
CGATCTG10000.033.955
ATCTGGG2350.032.7659577
TTCCGAT20200.032.0544552
CCGATCT20200.031.8811874
TCTGACC552.9864366E-431.818188
TAAAAGG552.9864366E-431.818183
CTTCCGA20600.031.432041
AACGGTG450.004301722631.11110970
CTAGTCT450.004301722631.1111094
ATAAGTC450.004301722631.11110955
ATGTTAT450.004301722631.11110922
TCGGTTA450.004301722631.11110933
CCTCTTA450.004301722631.1111091
Produced by FastQC (version 0.11.2)