FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96906
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3660.3776855922233917No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCAAT150.002218744770.013
TCTACTG150.002218744770.03
TACTAAT150.002218744770.07
AAGTAGC200.006932937552.536
ATCTACT200.006932937552.52
TGTGAGG200.006932937552.510
CATAACT200.006932937552.528
TTACGAG200.006932937552.538
AACACTT350.001250087940.025
TATCAGA459.139668E-538.8888851
AGGTGTA957.492417E-933.15789470
CAGAAGT552.9734135E-431.818184
GTATTAG450.00428920631.1111091
CTCCGCA450.00428920631.11110915
TATGGGA450.00428920631.1111092
TTATCGT450.00428920631.11110912
AGTGCGA450.00428920631.1111098
GCGGGTA450.00428920631.11110914
AAGTGCG450.00428920631.1111097
GTTATCG450.00428920631.11110911
Produced by FastQC (version 0.11.2)