FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences361913
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9530.26332295330645766No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC9460.26138878680787925No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC8290.22906057533164048No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC7740.21386355284281028No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC7590.2097189103458566No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC4920.13594427390008096Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA4820.13318117890211184Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGTGG200.006948207552.510
TTACCGT200.006948207552.54
TATAGAG552.9894328E-431.818185
ACTCTTA450.004304631.11110970
TGGACGT1052.0185325E-829.99999826
GTGTTAT500.00720394428.0000021
CGTATAG1004.41054E-728.00000230
GTCAATT500.00720394428.00000224
TTACAAT500.00720394428.00000239
GATTGTG657.948744E-426.92307951
GTCTAGG1056.7551264E-726.6666661
AACACGA1251.09586836E-725.19999916
AACCACT700.00122493125.033
GTTAATT700.00122493125.064
CTGTGCG700.00122493125.09
AGGGTGT1301.6012928E-724.2307735
AAGTGGA1301.6012928E-724.2307723
CTGTCCT1451.7211278E-824.13793228
CCACTTA750.001830616523.33333425
ACTGTTC750.001830616523.33333465
Produced by FastQC (version 0.11.2)