H3W35AFXY_n02_adr3wtzt14s18.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H3W35AFXY_n02_adr3wtzt14s18.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	330827
Sequences flagged as poor quality	0
Sequence length	76
%GC	54

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	737	0.22277504556762295	No Hit
ATTTAGAGCTGCATTCCCAAACAACTCGACTCGTCGAAGGAGCTTCACAT	563	0.17017958026400506	No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC	510	0.15415912244163868	No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC	454	0.13723184625196855	No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC	449	0.13572048230646228	No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC	377	0.11395684149117212	Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA	376	0.11365456870207086	Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC	373	0.11274775033476711	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGCTA	35	0.0012551658	39.99551	4
ATACGTG	45	9.1937734E-5	38.88452	67
TACGTGT	50	1.7074656E-4	35.00136	68
GGAGTCA	40	0.0024164908	35.001358	16
AACTCAT	40	0.0024164908	35.001358	31
TGGATAA	40	0.0024182838	34.996067	13
ATATTAT	40	0.0024182838	34.996067	1
CTATAGG	55	2.9908502E-4	31.81461	1
TAGTGTA	190	0.0	29.474833	70
ATGATTC	60	4.975578E-4	29.1678	69
ACTTGCC	60	4.9799844E-4	29.163393	38
TATGTTC	60	4.9799844E-4	29.163393	3
GTCTAGG	110	3.1745003E-8	28.633148	1
GGGTATT	75	5.574742E-5	28.00109	16
TCTAGGC	75	5.574742E-5	28.00109	8
CGTTATT	50	0.007201696	28.00109	10
TACTAAC	50	0.007201696	28.00109	48
GATACGT	50	0.007207013	27.996857	66
TAATATT	115	4.8876245E-8	27.388227	4
ATCATAC	65	7.945487E-4	26.924122	7