FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25043
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7302.9149862236952444No Hit
GACGTAATCAATGCGAGTTAATGACTCACACTTACTGGGAATTCCAAGTT490.19566345885077666No Hit
GTCCTGGGCTGCACGCGCGCTACAATGAAAGTATCAACGTGTATTTCCTA430.17170466797108971No Hit
GTTATTGCTCAATCTCATTATTGCTAGACGCAATTTGTCCATTTAAGAAG420.16771153615780857No Hit
ATTATAAGGACACTAGCTTCTTAAATGGACAAATTGCGTCTAGCAATAAT380.15173900890468395No Hit
TTCCTAGACCGAGAGGTCCGGGTAAACCGCTGAACCACTTTCATGCTTGG380.15173900890468395No Hit
GTCTAGGAAATACACGTTGATACTTTCATTGTAGCGCGCGTGCAGCCCAG370.14774587709140277No Hit
CACTTACTGGGAATTCCAAGTTCATGTGAACAGTTTCAGTTCACAATCCC360.14375274527812162No Hit
GGATTATGGTGCTGAAGCTTATGTAGCCTTCATTCATGTTGGCAGTAAAA340.13576648165155933No Hit
CTTCAGGATTATGGTGCTGAAGCTTATGTAGCCTTCATTCATGTTGGCAG330.13177334983827815No Hit
ATATTAAATAGATATCTTCAGGATTATGGTGCTGAAGCTTATGTAGCCTT330.13177334983827815No Hit
TAATGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCTGGGC320.127780218024997No Hit
TATGTAGCCTTCATTCATGTTGGCAGTAAAATGCTTATTGTGTTTGAATG260.10382142714531006No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCAC150.00219946470.065
GTCTTAC150.00219946470.01
CTTTCCG200.00687315152.563
TTTCTTA200.00687315152.519
CAACATC200.00687315152.510
TCCGATC503.3458182E-642.0000043
CTTCCGA503.3458182E-642.0000041
CCGATCT556.4403303E-638.1818164
TTATATA501.6631893E-435.0000044
TTCCGAT601.1697313E-535.02
Produced by FastQC (version 0.11.2)