FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53696
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4560.8492252681764005No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT910.1694725864123957No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG700.13036352800953516No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC610.11360250297973778No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG610.11360250297973778No Hit
GTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAA540.10056615017878427No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTTCG150.002213330270.07
AGCCTCG150.002213330270.05
TTTGACT150.002213330270.067
ACAAAGG200.006916148652.53
CATATAA200.006916148652.51
GGTATCA200.006916148652.51
CCCGGGC200.006916148652.513
CTAGGCG200.006916148652.59
CCCGCTT200.006916148652.553
TACAAAG200.006916148652.52
ATCTGGT451.6557133E-646.6666647
ACTATTA501.6888867E-435.0000043
ATCTAGC501.6888867E-435.0000047
TTAGAGT501.6888867E-435.00000421
TGAACAA501.6888867E-435.00000413
AGGCTTC652.0671054E-532.30769337
ATTAGAG552.9558482E-431.8181820
TAAAGCA552.9558482E-431.8181831
CTTCAAA552.9558482E-431.8181840
TTACTTT552.9558482E-431.818187