H3W35AFXY_n02_adr3wtzt14s15.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H3W35AFXY_n02_adr3wtzt14s15.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	308742
Sequences flagged as poor quality	0
Sequence length	76
%GC	55

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	1081	0.3501305296979355	No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC	678	0.21960083176244244	No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC	606	0.19628038945138662	No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC	517	0.1674537315946648	No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC	502	0.16259530611319484	No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA	447	0.14478107934780496	Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC	440	0.14251381412311898	Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGTA	35	2.967463E-7	60.003895	15
TTAGTTC	20	0.006949915	52.494896	27
TTACATA	45	1.6828835E-6	46.66213	28
TACATAA	50	3.47491E-6	41.99592	33
ACATAAA	50	3.47491E-6	41.99592	34
ACTCAGT	35	0.0012539421	40.002594	14
TACACGT	35	0.0012539421	40.002594	4
AACGTAT	45	9.182756E-5	38.891407	19
ATATAAG	55	6.672899E-6	38.19048	2
TATAAGA	55	6.680326E-6	38.18429	3
TATTCTA	40	0.002414013	35.00794	5
TAAAAGG	50	1.7069405E-4	35.00227	4
AGACGTA	50	1.7069405E-4	35.00227	67
TACCGCC	60	1.213131E-5	35.00227	7
TTACTAT	40	0.0024178543	34.996597	60
TACATAC	60	1.2144788E-5	34.996597	29
CATACAT	60	1.2144788E-5	34.996597	31
CTTACAT	60	1.2144788E-5	34.996597	27
TCGTATG	65	2.098137E-5	32.309788	9
ACATACA	65	2.1004638E-5	32.304554	30