FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423984
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGAGCTGCATTCCCAAACAACTCGACTCGTCGAAGGAGCTTCACAC10250.241754405826635No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8590.20260198498056528No Hit
GGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAA6510.15354352994452622No Hit
GTATTTAGCTTTAGATGAAATTTACCACCCATTTAGAGCTGCATTCCCAA5680.13396731952149138No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC5010.11816483640892109No Hit
GGCACGGACACCCCATCCCAGACGGGATTCTCACCCTCTCCGATGGCCCG4990.11769312049511303No Hit
GTCTAAGTGCCCTGGAACGGGCCATCGGAGAGGGTGAGAATCCCGTCTGG4900.11557039888297671No Hit
TTCCCAAACAACTCGACTCGTCGAAGGAGCTTCACACGGGCACGGACACC4600.10849466017585568No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC4400.10377750103777503No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG459.182132E-538.896035
TGGATTA654.8624315E-737.69034250
GGATTAA708.6862565E-734.99817351
TAGTTCT450.004301555531.1168254
TATACAG450.004301555531.1168255
TCCATCT450.004304039831.1131537
GATTAAC853.952857E-628.82202552
TGCACGC500.00719885828.0051422
GGTAGAA500.00719885828.00514220
TCTAATG657.947952E-426.9248498
ATACGAT657.9534313E-426.92167368
TAGCCAA1101.0137901E-625.4562210
CTAAAGA700.001223966425.0045914
CGTAAAT700.001225651124.99869323
TAAATTA700.001225651124.99869325
TCTGTGG1151.4932521E-624.3494268
CTTAAGG1451.7194907E-824.1423653
AGGTGTA3100.023.70843970
TATCATA1052.0338013E-523.337622
GACAGTT750.001829176123.3376217