FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences491546
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTATCCCCATCACGATGAAATTTCAAAGATTACCCGGACCTGTCGGC13150.26752328368046935No Hit
GGTGTGCACCGGTCGTCTCGTCCCTTCTGCCGGCGATGCGCTCCTGGCCT10980.2233768558792056No Hit
GGTATAGGTCGCGCGCTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTC10450.2125945486282057No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC10030.2040500787311869No Hit
GTCATAATCCAGCGCACGGTAGCTTCGCGCCACTGGCTTTTCAACCAAGC9670.1967262473908851No Hit
CCCTAACGTTGCCGTCAACCGCCGCGTCCCGGTTCAGGAATTTTAACCCG9000.18309578350754557No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTTACAAGGATTCCCCT8880.18065450639411165No Hit
ATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCGG8330.16946531962420608No Hit
ACACTGATGTATTCAACGAGTTTATAGCCTTGGCCGACAGGTCCGGGTAA8230.1674309220296778No Hit
GACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGGAATTCCTCGTT8210.16702404251077213No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7370.14993510271673455No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTTACAAGGATTCCC7220.14688350632494213No Hit
GGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGGAATT7020.14281471113588556No Hit
ACCTATACCCGGCCGTCGGGGCAAGCGCCAGGCCCCGATGAGTAGGAGGG6990.14220439185752706No Hit
GTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTTTATAGCC6810.13854247618737614No Hit
CTTCCGATCTGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTT6640.13508400027667808No Hit
CTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTT6230.1267429701391121No Hit
CCTATAAGCAACATCCGCCGATCCCTGGTCGGCATCGTTTATGGTTGAGA6160.1253188918229423No Hit
TTCCCAAACAACCCGACTCGCCGACAGCGCCTCGTGGTGCGACAGGGTCC6070.12348793398786685No Hit
GATGTATTCAACGAGTTTATAGCCTTGGCCGACAGGTCCGGGTAATCTTT6000.12206385567169704No Hit
CTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGT5640.11474002433139524No Hit
CATCAGTGTAGCGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACC5350.1088402713072632No Hit
ATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTC5210.10599211467492362No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT5210.10599211467492362No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCGG5200.10578867491547077No Hit
GTACTTGTTCGCTATCGGTCTCTCGCCCGTATTTAGCCTTGGACGGAATT5140.10456803635875381No Hit
ACGTAATAGGACCGAAATCCTATAATGTTATCCCATGCTAATGTATCCAG5130.10436459659930097No Hit
CTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAAT4940.10049924116969725No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGGAC200.00694301352.51274517
TACACCG407.4630043E-752.5127455
CACTAGG305.869074E-446.6779941
TAGTGTG305.877912E-446.6637510
ACGTGAT551.3112003E-744.54267569
ACACCGG459.197599E-538.886466
TAATACT601.213113E-535.0084954
CTGACGG400.002418832234.997819
CTGGCGG501.7096901E-434.997819
TGTCCGC400.002418832234.9978150
GAGTTGT3050.033.27660858
TGGGACG957.607014E-933.1659435
GGCCCTA851.0606345E-732.94917317
GTGAGTT3200.032.81044856
CTAGACT652.0980986E-532.3155364
TATACTG652.0980986E-532.3155365
AATACTA552.986723E-431.8259075
AGTGTGG552.9920766E-431.81619611
ATCTGGG6100.031.5554057
ATTCGGC2900.031.37734847
Produced by FastQC (version 0.11.2)