FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences422824
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC21260.5028096796775964No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC18090.42783758727035365No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC17760.42003292149925264No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC16480.3897602785083155No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9450.223497247081528No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC7610.1799803227820559Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA7450.17619624240818874Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTC5020.11872552173008155No Hit
GTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACG4550.10760978563184682No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACTC200.006949894652.49831836
AGAGGTT552.9908417E-431.81716265
ACAGCGT450.004303595531.1137928
TAGGGTC450.004303595531.1137924
TAAGGTA703.483631E-530.0025845
ACCTTTA604.976517E-429.1691788
TCGCTTA604.979964E-429.1657368
TACCGGT604.979964E-429.1657330
GTACTAT1451.8189894E-1128.9680141
GTCCTAG755.5765617E-528.0024151
TCCTAAG755.5765617E-528.0024152
TAAGTTA500.00720226528.002415
GTACCGA755.5810582E-527.9991026
ACCGGTG755.5810582E-527.99910231
GGACCGC500.007206424627.9991026
ACTATAC657.9469697E-426.9253963
GTCCTAA657.9469697E-426.9253961
AAGAATT657.9469697E-426.92539616
AAGCGTA657.9524633E-426.92221322
TTACCGG657.9524633E-426.92221329
Produced by FastQC (version 0.11.2)