FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences294054
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6180.21016547980983086No Hit
GTCCTGGGCTGCACGCGCGCTACAATGAAAGTATCAACGTGTATTTCCTA5350.18193937168003155No Hit
GACGTAATCAATGCGAGTTAATGACTCACACTTACTGGGAATTCCAAGTT4280.14555149734402525No Hit
TTCCTAGACCGAGAGGTCCGGGTAAACCGCTGAACCACTTTCATGCTTGG3640.12378678746080651No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTATA200.00694912252.495791
TAGTGTA1750.031.99743770
CGTTATC450.004301405531.11390742
ATGTCGT450.004304987431.10861818
ACAATTA450.004304987431.10861866
GGGTAGG802.4619076E-630.6225451
TAGGGGT1052.0172592E-829.9975974
ATCTGGG3750.029.8642717
TCTGGGC3050.029.8336738
TGGGCCG853.9482966E-628.8212210
GTCCTAA755.577189E-527.9977551
GAACCGT500.00720460227.9977556
TTTCGGA959.37267E-625.7874051
CACTGTG700.001224956624.9979977
GGGGGTT851.3064963E-424.70390316
TCTAGGC750.001830657123.3314638
GTGTACA1202.1613287E-623.3314631
TAAGGTA901.9251725E-423.3314635
TATACAT901.9251725E-423.3314633
GATCTGG9300.022.955156
Produced by FastQC (version 0.11.2)