FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences411972
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC4230.10267688095307449No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG450.004305832631.1103021
GACTATG500.00720627.9992717
TTTTGGC808.658957E-526.24931721
CCACAAT700.001224537525.00238614
GATCTGG12850.024.2406146
CGATCTG39650.023.4798435
CGCGGGA1202.1615324E-623.33555813
TAGAGTG1652.6502676E-923.3327275
CTAGACT750.001831321223.3327264
ATCTTGT1202.163817E-623.3327267
ATCTGGC3450.023.3327267
TCTGGAC1703.741661E-922.646473
CGATCTT17750.022.4782895
ATCTGGG6000.022.166097
GTAGGAA952.7754204E-422.10737212
CTGAGCG952.7777007E-422.1046879
TCTTAGC952.7777007E-422.1046878
CCTTATA800.002664673421.8744322
TAGGAGG1454.5949673E-721.7235744
CCGGGTA1957.748895E-1021.54051814
Produced by FastQC (version 0.11.2)