FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s96.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s96.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97
Sequences flagged as poor quality0
Sequence length76
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAAACCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTC22.0618556701030926No Hit
TGCGACTTCATCGTCAAAGCCATCACAGCTGAATACGCCGATGGGAAACC22.0618556701030926No Hit
TGTTCCTCCTATTTAAAAACCTGCATTAGTGCTCTTAAACGAGTGTTATT11.0309278350515463No Hit
CTTCATGGCATGAAAAAACAGTATTTTGCAAGCATCCGCTCAAAATGGGA11.0309278350515463No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACACCAA11.0309278350515463No Hit
TTTCATTGGTTTTCAGATCAAGAGGTAATGATTAATAGAAGCAGTTTTGG11.0309278350515463No Hit
GATCTGAAGAACGGCAGCGGCAGCCAGGCCAGCTTGGCGGTGGCGACCTC11.0309278350515463No Hit
GACTAACCCTGCGTCGATTAACGTTGCGCAGGAAACCTTGGTCTTTCGGC11.0309278350515463No Hit
CGTCTCCACGCCAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTA11.0309278350515463No Hit
CGTCTCCCTGGCAAGATCGGAAGAGCACACGTCTCCCTGGCAAGATCGGA11.0309278350515463Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CAAGAAAACATACAGAACGTATGTACTAGAACCCACGACACATATAAAAA11.0309278350515463No Hit
ATTGGAATGAGTACACTTTAAATCCTTTAACAAGGACCAATTGGAGGGCA11.0309278350515463No Hit
CCTCTGGCTTTTGGGGGGCGGAGGGCTTTTGCAGGACGCCACCAGCTCCC11.0309278350515463No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGAAAACCAC11.0309278350515463No Hit
GCACGCCCACGAGGGCGCCGTCGGGTCCCGTCACCACGTAGTCTGTCTCT11.0309278350515463No Hit
GCTTTGCAACCATACTTCCCCCGGAGCCCAAAAGCTTTGGTTTCCCGGGA11.0309278350515463No Hit
GAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATTGGTC11.0309278350515463No Hit
TGCTAGCTGGCATCGTTTATGGTTAGAACTAGGGCGGTATCTGATCGCCT11.0309278350515463No Hit
GGGTTGGACATGGGTTAGTCGATCCTAAGCCATAGGGAAGTTCCGTTTGA11.0309278350515463No Hit
CACTTACTGGGAATTCCAAGTTCATGTGAACAGTTTCAGTTCACAATCCC11.0309278350515463No Hit
CTTCGTTGGGGTTTCAGCTTTGCAACCATACTTCCCCCGGAGCCCAAAAG11.0309278350515463No Hit
CTAGGAATCTGCCTGGTAGTGGGGGATAACGTTCGGAAACGGACGCTAAT11.0309278350515463No Hit
GTATCGTCGCGGACCTCGGCGGCGAACAGCGACAACCCGGCCGCACCCTG11.0309278350515463No Hit
TCACATTGCCTTCGTGTTACGATGGAATTATTACAGCGATAGAGACATTA11.0309278350515463No Hit
GGCAAATGCTTTCGCTTAAGTTAGTCTTACGACGGTCCAAGAATTTCACC11.0309278350515463No Hit
GAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACC11.0309278350515463No Hit
AACCAAGAGCGTGAAAAATACAACCAGGTCGAATCGCTTCTCTGCAATGT11.0309278350515463No Hit
GCGGTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGATTAATGAA11.0309278350515463No Hit
GTGCTCTTCCGATCTGTGATGAGACGTGTGCTCTTCCGATCTGTGATGAG11.0309278350515463TruSeq Adapter, Index 1 (95% over 22bp)
GGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGG11.0309278350515463No Hit
GTCTTGATTTAATTGATTGGCTTCATTTAAATCATCTTCGCCACTTGTCG11.0309278350515463No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA11.0309278350515463No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC11.0309278350515463No Hit
ACGTTACCACGTTCCTAAAACGCAAGCTCTCCGGACCAGACGGACACAGG11.0309278350515463No Hit
CTTTCATGCTTGGGATTGTGAACTGAAACTGTTCACATGAACTTGGAATT11.0309278350515463No Hit
CGTCTCCCTCTCGAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGT11.0309278350515463No Hit
AAAAAAAAATAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAA11.0309278350515463No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC11.0309278350515463No Hit
GACGTGTGCTCTTCCGATCTGACAGGGGAGACGTGTGCTCTTCCGATCTG11.0309278350515463Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCAATGCGAGTTAATGACTCACACTTACTGGGAATTCCAAGTTCATGTG11.0309278350515463No Hit
GTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAG11.0309278350515463No Hit
GAACTGGCGTTCGCTGGCGCCGGGGTTGCCGGACACGAACACCGGCTCGC11.0309278350515463No Hit
GTACGACACCGAGCCCTTCTGCGGGTCCATGATCCCGCTGATCGTCTTCA11.0309278350515463No Hit
CTTCCGATCTCGTCAGGGGGCTGAAAAGCGTAGTCGATGGAAAACAGGTT11.0309278350515463No Hit
ACATAAGTGTGTAAGACAGATTGATAGCTCTTTCTCGAATCTATGGGTGG11.0309278350515463No Hit
ATGAAGATCGGAAGAGCACACGTCTCCCTATGAAGATCGGAAGAGCACAC11.0309278350515463Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCTTCAAGGGGGTGACGTGGTGCGCGGCCTTGATCGCCGCGTCGGTCGC11.0309278350515463No Hit
GTCCCCGTTCGCTCGCCACTACTAAGGGAATCTCGGTTGATTTCTTTTCC11.0309278350515463No Hit
GGAGACGTGTGCTCTTCCGATCTCCGTTGGGAGACGTGTGCTCTTCCGAT11.0309278350515463Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTTAGGGAAGGGGATGCGGAACTCGGCCGCTACCGGGAGTACACCCAG11.0309278350515463No Hit
CGGAAGAGCACACCCCCTGTTAGATCGGAAGAGCACACGTCTCCCTGTCT11.0309278350515463Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCTGACCTGGAGACGTGTGCTCTTCCGATCTGGTCAGGGAGACGTGTGC11.0309278350515463Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GGATGAGGACGATGGCAGCGGTGCTGACCGGCGCGGCCGGCGCCTCGGCC11.0309278350515463No Hit
GTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCCCCCAAACTGCT11.0309278350515463No Hit
CGTCTCCCTGGCTAGATCGGAAGAGCACACGTCTCCCTGGCTAGATCGGA11.0309278350515463Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GCGCCAGGACCAGCGCGGGCAGGGGGCGGATGAAAGGCATGGATCCTCAC11.0309278350515463No Hit
CGTCTCCCCAAATAGATCGGAAGAGCACACGTCTCGGAAGAGCACACGTC11.0309278350515463Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GAGCACCCCCTCCGAGATCGGAAGAGCACCTGTCTCTTATACGCATCTCC11.0309278350515463No Hit
CTTCTATCCATGAAGGGACTCAACGGGGACCTACCCCCGACGTTCACGAC11.0309278350515463No Hit
TCTTTACTCGTAGTGCGATTTCACCGGGCCTGTGGTTGGGACAGCAGAGA11.0309278350515463No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT11.0309278350515463No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT11.0309278350515463No Hit
TCATTGTAGCGCGCGTGCAGCCCAGGACATCTAAGGGCATCACAGACCTG11.0309278350515463No Hit
GCCTGCTGATTGCCTGCCTGCTGCCCATTGGCTGCACCCTGATCGCCAAG11.0309278350515463No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA11.0309278350515463No Hit
CGTCACTACCTCCCCGAGCTGGGAGTGGGTAATTTACGCGCCTGCTGCCT11.0309278350515463No Hit
GTGCAGTAAGGCTCTGATTTATGACTTTTTAAGTAGGCAATTTTGTAAAA11.0309278350515463No Hit
GATATGAGTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGAGCTGGGA11.0309278350515463No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAGAACAAC11.0309278350515463No Hit
GTATTGTACCGGCGATCAGGATGAGCGCCTCGCAGAGCCTGTATCTTATA11.0309278350515463No Hit
GATCAAACCCCAGAACGGCAGCGTGGCAGGCGTCGCGATCTTGACGAAGA11.0309278350515463No Hit
GGCGCTGATCCGGCAGCTGGGGTGCAGCCATATCCAGGGCTATGTCTATG11.0309278350515463No Hit
GCGCAGGATCATCAGCGCGGTGTGCCCGAGGTCGCGCACCAGGCGGGCCT11.0309278350515463No Hit
TCTCCCGTCCCAGATCGGAAGAGCACACGTCTCCCCGGAAGAGATCGGAA11.0309278350515463Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GTGCGGCGGTGGAGTGAGCGTTCGCCTGTCCTAGAAGTCCAATTTCAACA11.0309278350515463No Hit
GGCTCATTATATCAGTTATGGTTCCTTAGATCGTTAACAGTTACTTGGAT11.0309278350515463No Hit
CTTGGCTACGAGCTGAAGCCCGTGCCTGTATCTCCTACCGGACGACTCAA11.0309278350515463No Hit
GTGTATCTGTGTCTGAGTGTGTGTGTGTGTGTGTGTGTCTGTCATTTGTT11.0309278350515463No Hit
GTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGATCT11.0309278350515463No Hit
GAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCTGGGCTGCACGCGCG11.0309278350515463No Hit
CTATAATTCAATCAATAGCGTATAAATTAAAAAATATATCAATGAATAAC11.0309278350515463No Hit
GGGCTGTCGGGCATCCGCACCGACACCTGGTCCTACTCGGACCGCGACTT11.0309278350515463No Hit
GTGCTCTTCCGATCTACAGTGGGAGACGTGTGCTCTTCCGATCTACAGTG11.0309278350515463Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCTGAAGAGATCGGAAGAGCACACGTCTCCCTGAAGAGATCGGAAGAGC11.0309278350515463Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CGACTGGGTGGGGAAGTGAAACATCTCAGTACCTACAGGAAGAGAAAACA11.0309278350515463No Hit
CTTCCGATCAGGATCTCCGGAAAGTTCCGTGCGGCAGGATCACCGACGAA11.0309278350515463No Hit
GCATATTTCATAACAAAATCTATACTCACATCTTCAGTTTTGTAGTAGTA11.0309278350515463No Hit
AATCCACACAAATACACAGAAACATTGGACATGTGGAGGAGACAACATCG11.0309278350515463No Hit
GCAATAATGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCT11.0309278350515463No Hit
TCCTCGTCCAGGTCACTGGAGTCCACCAGGTCATCCATAACACCGGACAC11.0309278350515463No Hit
GTTTTATGTGCTGCCCAAGCACCTGGACGAGAAGGTCGCACGCCTGCAGC11.0309278350515463No Hit
AGTAAGGAAACAATAAGAGTAGTGGTATTTCATTGACGATACCAAACCGA11.0309278350515463No Hit
ACCTAAGGAGAGGCAATTCGATAGAGCAAGTACATTGCATTTATCGCCGA11.0309278350515463No Hit
TCTTTTTTTTTTTTTTTTTTTTTTTTTTGGGAAACGCCCCCCCCGGGGGG11.0309278350515463No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTGGGAAAACAC11.0309278350515463No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTCGT50.070.01
ACCGGAC50.070.042
GTCACTG50.070.012
CCGGACA50.070.043
GTCATCC50.070.030
ATCCATA50.070.033
AAATCTT50.070.070
AGAAGTT50.070.052
CCAAATC50.070.068
CAACTTC50.070.062
CCTCGTC50.070.02
GAAGTTC50.070.053
GTTCCAC50.070.056
CACAACT50.070.060
CACCGGA50.070.041
CATCCAT50.070.032
ACACCGG50.070.040
CCAGAAG50.070.050
ACACCAG50.070.047
GGTCATC50.070.029