FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s95.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s95.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences293707
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC5890.20053999393953836No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC5590.19032573278811876No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC5190.17670671791955928No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC3920.13346634571188293No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC3620.12325208456046331Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT3610.12291160918874934No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA3370.11474020026761365Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACT200.00694704552.4997444
TTGTAAC200.00694704552.4997443
CAATTAC200.00694704552.49974438
TATTAGA200.00694704552.4997442
ATAAACT200.00694704552.49974451
GACTGCT200.00694704552.4997447
TAGACTG350.001254272939.9998055
GTATTAA400.002412535335.011751
TCATAGG400.002412535335.011751
AGCCAAA400.002416569734.9998311
TCTAACA552.9881558E-431.8180272
ATTTACT450.004303393431.1109627
AATTTAC450.004303393431.1109626
CTGTAGT450.004303393431.1109624
TCTAGGG450.004303393431.1109628
TTTCGGA1251.03682396E-1030.8103391
TGCTTTA604.975511E-429.16652335
TTAATTT500.00720193927.9998654
TTGTACG500.00720193927.9998653
ACTAGGC500.00720193927.99986535