Basic Statistics
Measure | Value |
---|---|
Filename | H3W35AFXY_n01_adr3wtzt14s95.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 293707 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 56 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC | 589 | 0.20053999393953836 | No Hit |
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC | 559 | 0.19032573278811876 | No Hit |
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC | 519 | 0.17670671791955928 | No Hit |
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC | 392 | 0.13346634571188293 | No Hit |
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC | 362 | 0.12325208456046331 | Illumina Multiplexing PCR Primer 2.01 (95% over 22bp) |
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT | 361 | 0.12291160918874934 | No Hit |
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA | 337 | 0.11474020026761365 | Illumina Multiplexing PCR Primer 2.01 (95% over 21bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CTAGACT | 20 | 0.006947045 | 52.499744 | 4 |
TTGTAAC | 20 | 0.006947045 | 52.499744 | 3 |
CAATTAC | 20 | 0.006947045 | 52.499744 | 38 |
TATTAGA | 20 | 0.006947045 | 52.499744 | 2 |
ATAAACT | 20 | 0.006947045 | 52.499744 | 51 |
GACTGCT | 20 | 0.006947045 | 52.499744 | 7 |
TAGACTG | 35 | 0.0012542729 | 39.999805 | 5 |
GTATTAA | 40 | 0.0024125353 | 35.01175 | 1 |
TCATAGG | 40 | 0.0024125353 | 35.01175 | 1 |
AGCCAAA | 40 | 0.0024165697 | 34.99983 | 11 |
TCTAACA | 55 | 2.9881558E-4 | 31.818027 | 2 |
ATTTACT | 45 | 0.0043033934 | 31.110962 | 7 |
AATTTAC | 45 | 0.0043033934 | 31.110962 | 6 |
CTGTAGT | 45 | 0.0043033934 | 31.110962 | 4 |
TCTAGGG | 45 | 0.0043033934 | 31.110962 | 8 |
TTTCGGA | 125 | 1.03682396E-10 | 30.810339 | 1 |
TGCTTTA | 60 | 4.975511E-4 | 29.166523 | 35 |
TTAATTT | 50 | 0.007201939 | 27.999865 | 4 |
TTGTACG | 50 | 0.007201939 | 27.999865 | 3 |
ACTAGGC | 50 | 0.007201939 | 27.999865 | 35 |