H3W35AFXY_n01_adr3wtzt14s94.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H3W35AFXY_n01_adr3wtzt14s94.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	245585
Sequences flagged as poor quality	0
Sequence length	76
%GC	53

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC	396	0.16124763320235358	No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC	388	0.15799010525887167	No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC	376	0.15310381334364884	No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC	334	0.1360017916403689	No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA	287	0.11686381497241281	Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC	280	0.11401347802186615	Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTATAT	15	0.002222841	69.999794	8
GACGTTT	20	7.91634E-5	69.999794	57
TAGACCA	20	0.006945641	52.499847	5
CTAGTAG	20	0.006945641	52.499847	3
CTAGCAG	20	0.006945641	52.499847	9
AACTATA	55	6.6778557E-6	38.18171	7
GCCTTAA	40	0.0024133644	35.007027	1
TTCGTTA	55	2.986704E-4	31.81809	23
ACGTTTG	45	0.004301991	31.111023	58
TAGACAG	45	0.004301991	31.111023	5
TATGACT	45	0.004301991	31.111023	4
AGACGTT	45	0.004301991	31.111023	56
CATAGTG	45	0.004301991	31.111023	48
ACTCAAC	45	0.004301991	31.111023	62
ACATAAC	45	0.004301991	31.111023	3
TGTGTTA	45	0.004301991	31.111023	30
CTTACAC	110	3.164132E-8	28.636282	3
GTTTAAT	50	0.0071924496	28.005623	1
GTCCTAG	50	0.0071924496	28.005623	1
GTATATG	50	0.0071924496	28.005623	1