FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s92.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s92.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences218982
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT5490.25070553744143353No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2340.10685809792585692No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCCA350.00125354440.05
AGTGTCG350.00125354440.026
TATTAAT459.177513E-538.888892
TCAGGGG556.674407E-638.1818163
TCTAGGC601.2120625E-535.08
TTTCGGA1350.033.7037051
TCTAGCG450.004300920831.1111138
TCTACTC450.004300920831.1111138
ACTCCTA450.004300920831.11111329
TCTTTTT3800.029.4736828
TGAACGG604.9712823E-429.1666665
TCCTGTA1103.160676E-828.6363622
ATCTAGC1004.3971158E-728.0000027
CCGTCTT1004.3971158E-728.00000247
GTCAAGA500.007197825728.00000213
GCGATAA500.007197825728.00000238
ATTGGAC500.007197825728.0000023
ACTCCAT500.007197825728.0000026
GGACAAT500.007197825728.0000026
CTTTTTT4050.027.654329
Produced by FastQC (version 0.11.2)