FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s91.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s91.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences227133
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT3470.15277392540934165No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTATG207.914947E-570.02
ACTATTC200.006944892552.58
CTAGTGG200.006944892552.53
ACCCTTC305.869389E-446.6666647
ATCTAGT350.001253642640.07
GTGCTAT350.001253642640.01
GACCGTT654.8471884E-737.6923077
ACCGTTA654.8471884E-737.6923078
CTCGTAT852.559318E-937.05882339
GTATGCC904.476533E-935.00000442
GTATTGG501.7061738E-435.01
TTATGAG400.002415361635.03
GGGCGAC400.002415361635.010
TTTCGGA1650.033.9393961
CTTCTGC957.587005E-933.15789851
ATACTGG552.985951E-431.818186
GGTAGGA450.004301255631.1111132
TTCGGAA1950.030.5128212
CCGTCTT1202.2810127E-929.16666647
TCTTCTG1103.1617674E-828.63636250