FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s90.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s90.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239816
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT6000.25019181372385496No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGTA150.002222752270.019
ACTCGAG150.002222752270.038
TTCTAAT200.006945363252.51
CTTTTAC200.006945363252.567
ATGCTAC200.006945363252.56
TATAACT200.006945363252.53
ATCTGTT459.1801325E-538.8888857
TTTCGGA1400.037.5000041
TAAGAGT400.0024156335.04
TCTAATA652.0971263E-532.3076932
GGTCCCT802.4578694E-630.62525
GTATGCC1151.4479156E-930.43478442
ATCTGGT1650.029.696977
CTCGTAT1202.2828317E-929.16666639
GATCTGG6400.028.9843756
CGATCTG14400.028.9236095
GCAAGTG500.00719917328.00000250
CTTTTAT500.00719917328.00000217
TGATTCC500.00719917328.0000024
GAACGTA500.00719917328.00000218
Produced by FastQC (version 0.11.2)