H3W35AFXY_n01_adr3wtzt14s88.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H3W35AFXY_n01_adr3wtzt14s88.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	125896
Sequences flagged as poor quality	0
Sequence length	76
%GC	53

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC	554	0.4400457520493105	No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC	425	0.3375802249475758	No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC	396	0.3145453390099765	No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC	386	0.30660227489356295	No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA	335	0.2660926478998538	Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC	322	0.25576666454851626	Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	128	0.1016712206900934	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCCACT	15	0.0022202907	70.00001	52
CTATGGC	20	0.006937734	52.5	14
GCGTTTT	20	0.006937734	52.5	63
GCAATCA	20	0.006937734	52.5	14
ACCCACT	20	0.006937734	52.5	9
GCCACTT	35	0.001251513	40.000004	53
AGCCGTT	40	0.0024112843	35.0	19
ACGTCAC	40	0.0024112843	35.0	68
CTCAAGA	45	0.0042940388	31.111109	4
TTTGAGT	45	0.0042940388	31.111109	67
CCCTAAT	60	4.9594115E-4	29.166668	1
CGCAGCT	50	0.0071863784	27.999998	48
GGCTAGA	50	0.0071863784	27.999998	20
CTTTTTT	205	0.0	25.609756	14
GCGGGAG	70	0.0012204933	25.000002	14
ATCTTTT	210	0.0	24.999998	12
TCTTTTT	235	0.0	22.340425	13
ATCTACG	80	0.0026541	21.875	7
GTCTACC	80	0.0026541	21.875	70
ATCTGGG	170	2.0894277E-6	18.529411	7