FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s87.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s87.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences394271
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT8860.224718531162576No Hit
CGTTGAGACAGTGCCCAAATCGTTACGCCTTTCGTGCGGGTCGGAACTTA8230.20873967398058693No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAA6760.1714556738892792No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG5980.15167232690205468No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC5320.13493257175901854No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT5260.13341077583692434No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA5230.13264987787587726No Hit
GTATAGGGGCTGACGCCTGCCCGGTGCTGGAAGGTTAAGAGGAGTGGTTA5160.1308744493001007No Hit
GAATAGGCCCAAGCGACTGTTTATCAAAAACACAGGTCTCTGCTAAACCG5110.12960628603168886No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA5110.12960628603168886No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT4970.12605542888013574No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC4840.12275820438226501No Hit
ATTCTAAGGTGAGCGAGCGAACTCTCGTTAAGGAACTCGGCAAAATGACC4790.12149004111385316No Hit
TTCCAGCACCGGGCAGGCGTCAGCCCCTATACATCACCTTACGGTTTAGC4600.11667102069388821No Hit
GTGCTGGAAGGTTAAGAGGAGTGGTTAGCTTCTGCGAAGCTACGAATCGA4320.10956930639078198No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAG4310.10931567373709962No Hit
GTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAA4210.10677934720027596No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT4210.10677934720027596No Hit
CATCTAAGGAAGGCAGCAGGCGCGTAAATTACCCACTCCCAGCTCGGGGA4170.1057648165855465No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG4110.10424302066345228No Hit
GGTACTATGATTTCACCGAGTCTCTCGTTGAGACAGTGCCCAAATCGTTA4050.1027212247413581No Hit
GTTTAGCAGAGACCTGTGTTTTTGATAAACAGTCGCTTGGGCCTATTCAC3990.1011994288192639No Hit
CCTTAGAATTCTCATCTTGACTACCTGTGTCGGTTTGCGGTACGGGCACC3970.10069216351189918No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATTGT350.001254765540.023
GGTATAC501.7084501E-435.06
TGAGAAC400.002417512535.056
TTAAGGT957.617928E-933.1578984
TATTTCT450.004305062331.11110949
TCAATCC450.004305062331.11110935
CCATATA450.004305062331.1111092
GCCTTAT604.978425E-429.1666661
GTTACAC604.978425E-429.16666662
TACACCG500.007204715627.9999985
TTATGAG657.9500146E-426.9230774
TAGTTGC657.9500146E-426.92307740
GCAGGGG657.9500146E-426.92307713
CGCGTAA1705.456968E-1226.76470421
GGAGTCA808.6565014E-526.2570
CGTAAAT1606.548362E-1126.2523
GGCTAGT808.6565014E-526.2537
TAGGTAT700.001225125325.04
AGTGCAC700.001225125325.029
TAGATAT700.001225125325.09
Produced by FastQC (version 0.11.2)