FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s86.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s86.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences245902
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC5110.2078063618840026No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC4340.1764930744768241No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC4230.17201974770437004No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC4070.1655130905808005No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT3000.1219998210669291No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAG200.0069425452.5057953
TACATCC200.00694812752.4951220
TTTCGGA701.9159415E-840.0044171
TCTATAG400.002414440735.0038643
TCTATAC400.002414440735.0038643
GCTAACC400.002416851234.99674663
CCTTATA552.9847305E-431.8216932
GTTTACC755.558577E-528.00878729
TAGATAC755.56629E-528.0030925
TGAGTCG500.00720279127.99739610
TCTTTTT3150.026.6641888
CTTTTTT3200.026.247569
ATTAATA959.35067E-625.7923222
ATCTTTT3450.025.359967
CATAGGT700.00122302625.002764
GCGGGTA700.00122302625.0027614
AGTGTGT700.001224477324.9976758
ATCTAGC1451.717126E-824.1356877
CCGTTTA1052.0272584E-523.34065627
CTATACA750.001827779723.335914
Produced by FastQC (version 0.11.2)