Basic Statistics
Measure | Value |
---|---|
Filename | H3W35AFXY_n01_adr3wtzt14s85.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 224589 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 56 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC | 640 | 0.2849649804754462 | No Hit |
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC | 572 | 0.2546874512999301 | No Hit |
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC | 532 | 0.2368771400202147 | No Hit |
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC | 444 | 0.19769445520484083 | No Hit |
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT | 344 | 0.15316867700555237 | No Hit |
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC | 244 | 0.10864289880626388 | Illumina Multiplexing PCR Primer 2.01 (95% over 22bp) |
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA | 231 | 0.10285454764035638 | Illumina Multiplexing PCR Primer 2.01 (95% over 21bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CTCTAGG | 20 | 0.0069387634 | 52.511528 | 1 |
TGACACC | 20 | 0.0069448785 | 52.499832 | 18 |
CTCGTAT | 60 | 4.9112714E-9 | 46.666515 | 39 |
GCGAATC | 35 | 0.0012536319 | 39.999874 | 67 |
TACATGG | 35 | 0.0012536319 | 39.999874 | 2 |
TGTAAGT | 35 | 0.0012536319 | 39.999874 | 4 |
TCGTATG | 75 | 3.520654E-8 | 37.333218 | 40 |
GTACCGA | 40 | 0.0024153418 | 34.99989 | 6 |
GTTTAGG | 40 | 0.0024153418 | 34.99989 | 45 |
ACGGATT | 40 | 0.0024153418 | 34.99989 | 16 |
GTATGCC | 85 | 1.0585609E-7 | 32.94107 | 42 |
TCTAGCG | 45 | 0.004301221 | 31.111012 | 8 |
CCGTCTT | 95 | 2.807592E-7 | 29.473593 | 47 |
AAAGGGG | 95 | 2.807592E-7 | 29.473593 | 68 |
TTTCGGA | 60 | 4.965295E-4 | 29.173069 | 1 |
GCTTAGG | 50 | 0.0071905004 | 28.006147 | 1 |
TCTTCTG | 100 | 4.398098E-7 | 27.99991 | 50 |
TCTGTTA | 50 | 0.0071983244 | 27.99991 | 27 |
GTCTTCT | 100 | 4.398098E-7 | 27.99991 | 49 |
GCTCCTA | 50 | 0.0071983244 | 27.99991 | 52 |