FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s85.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s85.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224589
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC6400.2849649804754462No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC5720.2546874512999301No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC5320.2368771400202147No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC4440.19769445520484083No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT3440.15316867700555237No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC2440.10864289880626388Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA2310.10285454764035638Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAGG200.006938763452.5115281
TGACACC200.006944878552.49983218
CTCGTAT604.9112714E-946.66651539
GCGAATC350.001253631939.99987467
TACATGG350.001253631939.9998742
TGTAAGT350.001253631939.9998744
TCGTATG753.520654E-837.33321840
GTACCGA400.002415341834.999896
GTTTAGG400.002415341834.9998945
ACGGATT400.002415341834.9998916
GTATGCC851.0585609E-732.9410742
TCTAGCG450.00430122131.1110128
CCGTCTT952.807592E-729.47359347
AAAGGGG952.807592E-729.47359368
TTTCGGA604.965295E-429.1730691
GCTTAGG500.007190500428.0061471
TCTTCTG1004.398098E-727.9999150
TCTGTTA500.007198324427.9999127
GTCTTCT1004.398098E-727.9999149
GCTCCTA500.007198324427.9999152