FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s84.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s84.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences320618
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT10080.3143928288492848No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG7300.22768528279759714No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA6540.2039810615748336No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT6380.19899069921214654No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT6190.19306464390645564No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA6170.1924408486111198No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC6100.19025756507744418No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC5820.18152443094274184No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC5670.17684596622772272No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC5540.17279129680803948No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC5430.1693604226836921No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT5030.15688451677697446No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG4620.14409671322258888No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT4610.14378481557492093No Hit
CATCTAAGGAAGGCAGCAGGCGCGTAAATTACCCACTCCCAGCTCGGGGA4590.14316102027958505No Hit
GTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAA4580.14284912263191712No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAG4540.14160153204124534No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA4250.13255650025887505No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC4160.1297494214298636No Hit
GCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATGAA3980.12413526377184063No Hit
CATATAAGAACTCCACCGGTAATACGCTTACATACATAAAGGTATAGTAC3940.12288767318116887No Hit
CTTTAAATCCTTTAACAAGGACCAATTGGAGGGCAAGTCTGGTGCCAGCA3840.11976869670448946No Hit
GAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGA3650.11384264139879857No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3640.11353074375113063No Hit
GTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCAAC3630.11321884610346268No Hit
ATACAGGACTCATATCCGAGGCCCTGTAATTGGAATGAGTACACTTTAAA3490.10885227903611151No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGAGCTGGGAGTGGGTA3450.10760468844543974No Hit
CCTTATGGGACATGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGAT3400.10604520020710004No Hit
CATTATATCAGTTATGGTTCCTTAGATCGTTAACAGTTACTTGGATAACT3250.10136673549208092No Hit
TCATTATCCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCA3230.10074294019674504No Hit
TTCTAGAGCTAATACATGCAATTAAAACATGAACCTTATGGGACATGTGC3220.10043104254907709No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGTA252.3867456E-456.0079885
TAGGGTT200.006943572352.5074844
TATAGAC200.006943572352.5074842
AGTTACC200.00694785752.49929417
TAGGGGT501.7063074E-435.0049934
GTATAGA501.7063074E-435.0049931
GCCCATA400.00241734.9995337
ACAAACG400.00241734.9995351
ATATAAG1600.032.817182
TTTCGGA652.097273E-532.3122981
TAGGACC552.9861764E-431.822724
TATAAGA1550.031.617413
GTTCCAA450.00430087131.115551
CATGGAT450.004304155731.11069537
TAATAAC450.004304155731.11069546
ACTACTC450.004304155731.11069532
TACTCAA450.004304155731.11069560
CATATAA1800.029.1708261
ACCGGAC604.9767643E-429.16627525
TACATTC604.9767643E-429.1662757
Produced by FastQC (version 0.11.2)