FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s83.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s83.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187774
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGTAATCAATGCGAGTTAATGACTCACACTTACTGGGAATTCCAAGTT6110.3253911617156795No Hit
GTCCTGGGCTGCACGCGCGCTACAATGAAAGTATCAACGTGTATTTCCTA5570.29663318670316446No Hit
CACTTACTGGGAATTCCAAGTTCATGTGAACAGTTTCAGTTCACAATCCC4690.24976833853462144No Hit
GTCTAGGAAATACACGTTGATACTTTCATTGTAGCGCGCGTGCAGCCCAG4510.2401823468637831No Hit
TTCCTAGACCGAGAGGTCCGGGTAAACCGCTGAACCACTTTCATGCTTGG4220.22473824917187682No Hit
GACTCACACTTACTGGGAATTCCAAGTTCATGTGAACAGTTTCAGTTCAC4090.21781503296516022No Hit
TAATGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCTGGGC3790.20183838018042966No Hit
TTCCAAGTTCATGTGAACAGTTTCAGTTCACAATCCCAAGCATGAAAGTG3520.18745939267417214No Hit
GTTTCAGTTCACAATCCCAAGCATGAAAGTGGTTCAGCGGTTTACCCGGA3250.17308040516791462No Hit
GATAAGGACACTAGCTTCTTAAATGGACAAATTGCGTCTAGCAATAATGA3100.16509207877554935No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC3090.16455952368272497No Hit
CTTCCGATCTGGGCAGGGACGTAATCAATGCGAGTTAATGACTCACACTT3080.16402696858990062No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC2940.15657119729035968No Hit
TAACAGGTCTGTGATGCCCTTAGATGTCCTGGGCTGCACGCGCGCTACAA2930.15603864219753533No Hit
GGGATAAGGACACTAGCTTCTTAAATGGACAAATTGCGTCTAGCAATAAT2840.15124564636211615No Hit
GGACACTAGCTTCTTAAATGGACAAATTGCGTCTAGCAATAATGAGATTG2830.1507130912692918No Hit
GAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCTGGGCTGCACGCGCG2670.14219220978410216No Hit
CTTACTGGGAATTCCAAGTTCATGTGAACAGTTTCAGTTCACAATCCCAA2650.14112709959845346No Hit
TCACAATCCCAAGCATGAAAGTGGTTCAGCGGTTTACCCGGACCTCTCGG2640.1405945445056291No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC2640.1405945445056291No Hit
GGATAAGGACACTAGCTTCTTAAATGGACAAATTGCGTCTAGCAATAATG2550.13580154867020994No Hit
CCTTAGATGTCCTGGGCTGCACGCGCGCTACAATGAAAGTATCAACGTGT2440.12994344264914204No Hit
ACAATGAAAGTATCAACGTGTATTTCCTAGACCGAGAGGTCCGGGTAAAC2400.12781322227784464No Hit
GCAATAATGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCT2360.12568300190654724No Hit
GCATGAAAGTGGTTCAGCGGTTTACCCGGACCTCTCGGTCTAGGAAATAC2340.12461789172089853No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC2310.12302022644242547No Hit
CTACAATGAAAGTATCAACGTGTATTTCCTAGACCGAGAGGTCCGGGTAA2310.12302022644242547No Hit
GGGCAGGGACGTAATCAATGCGAGTTAATGACTCACACTTACTGGGAATT2220.1182272306070063No Hit
CATGTGAACAGTTTCAGTTCACAATCCCAAGCATGAAAGTGGTTCAGCGG2150.11449934495723582No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC2110.11236912458593841No Hit
AGCAATAACAGGTCTGTGATGCCCTTAGATGTCCTGGGCTGCACGCGCGC2090.11130401440028971No Hit
GGTCTGTGATGCCCTTAGATGTCCTGGGCTGCACGCGCGCTACAATGAAA2080.11077145930746535No Hit
TAATCAATGCGAGTTAATGACTCACACTTACTGGGAATTCCAAGTTCATG2040.10864123893616795No Hit
GTATCAACGTGTATTTCCTAGACCGAGAGGTCCGGGTAAACCGCTGAACC1980.10544590837922184No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC1960.10438079819357311No Hit
CCCTTAGATGTCCTGGGCTGCACGCGCGCTACAATGAAAGTATCAACGTG1890.10065291254380267No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACGA150.002221998370.055
ATGCTAA200.00694302652.511
AACTATT200.00694302652.518
GTACAAA556.669323E-638.181821
AGGTCGA400.002414298735.056
TCTGGAC552.9839916E-431.8181848
ATCCGGG450.00429937431.11111312
GCACAAG450.00429937431.11111354
TAGGGGT450.00429937431.1111134
TCTTTTT3750.030.8000018
GGTCCAA802.454115E-630.62511
ATCTTGC1151.4442776E-930.4347847
ATCTGGG2300.030.4347847
ATCTTTT4150.029.518077
CTTTTTT3950.029.2405079
GATCTTT4550.029.230776
TGGGTGG604.9686135E-429.1666665
GGAGTGG500.007195254327.99999822
ATCTTGT1154.8587026E-827.3913067
GATCTTG2650.026.4150926
Produced by FastQC (version 0.11.2)