FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s82.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s82.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207992
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT4450.21395053655909846No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT3090.14856340628485712No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA2960.1423131658909958No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA2930.14087080272318167No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT2730.13125504827108733No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA2470.11875456748336474No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC2430.1168314165929459No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC2410.11586984114773645No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG2280.10961960075387515No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG2260.10865802530866572No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTCTA150.002222366269.9997643
TATATAC200.006944166552.4998173
TATAAGT200.006944166552.4998172
ACTTATG459.176112E-538.88875210
TCTTTTT1950.035.89731213
CTAACGT400.002414935334.9998784
ATCTTGT400.002414935334.9998787
ATCTGTG904.472895E-934.9998787
CCGATCT18900.033.5184064
ATCTTTT2200.033.40897812
ACAATAC751.4841062E-632.66655333
CTTTTTT2150.032.55802514
GATCTGG4000.032.3748866
TCCGATC19750.032.075843
ATCTATG552.9851505E-431.8180717
GCTCTAC552.9851505E-431.81807113
ATCTGGG1550.031.6127957
TTCCGAT20200.031.3612772
CTTCCGA20250.031.2913681
TATGCTT901.7470484E-731.1110025
Produced by FastQC (version 0.11.2)