FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s81.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s81.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences266457
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT4460.16738160378597672No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT2990.11221322765024No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA2910.1092108670442135No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT2790.10470732613517376No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA2670.10020378522613405No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGATC150.002221535270.0118263
TCTAGGC459.183897E-538.8881578
AGTAGTA459.183897E-538.88815725
TCCGATC19900.038.1722763
CCGATCT19600.037.8635374
TTCCGAT20150.037.524952
CTTCCGA20300.037.2546651
GATCTAG1300.035.0059136
TTACAGT501.7052713E-435.0059134
CTGAGCG501.7071543E-434.9993449
CGATCTG9750.034.8263975
ATCTGTG1350.033.703077
CGATCTA3650.032.608255
TTTACAG552.9843708E-431.8235593
TCTGTCA450.004302976731.1105258
TTCTGAT450.004302976731.11052521
TCTGAGC952.811321E-729.4731338
TGAGCGG604.9746915E-429.16611910
TCTTACA853.938818E-628.82842
ATCTGAG1103.1666787E-828.6358287
Produced by FastQC (version 0.11.2)