FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s80.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s80.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences315303
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT5320.16872658997852857No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG4360.13827968652375652No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC4260.13510813408055108No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC4260.13510813408055108No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA4030.12781356346117861No Hit
TCATTATCCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCA3910.12400770052933209No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA3910.12400770052933209No Hit
CATATAAGAACTCCACCGGTAATACGCTTACATACATAAAGGTATAGTAC3800.12051899284180613No Hit
GTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAA3730.11829890613156233No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT3630.1151273536883569No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT3620.11481019844403638No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA3440.10910140404626661No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAG3330.10561269635874064No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT3240.10275829915985576No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGTCA459.186838E-538.88889
ACTATCG552.9886054E-431.81810811
GAGTATT450.00430381531.1110427
GGTGGGT450.00430381531.111048
TGGATTA450.00430381531.1110418
CTCGTTT952.8136856E-729.47361826
CACTATC604.9762556E-429.166610
CCCGATT604.9762556E-429.166664
TCTCTGA500.007202639227.9999378
CGTTATT500.007202639227.99993711
TAGATTC500.007202639227.99993716
ATAACAC1004.407575E-727.99993720
ATAGGAG906.1570645E-627.2221657
AGAAACG1950.026.92301867
TTTCGGA959.35828E-625.7935051
AAATAGG959.369811E-625.78941555
ACATATG959.369811E-625.78941566
AAAGTAA1101.0130098E-625.45448764
AGGTTTT1101.0130098E-625.45448747
GAGAAAC2200.025.45448766
Produced by FastQC (version 0.11.2)