FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74539
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC2410.3233206777660017No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC2260.30319698412911367No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC2190.29380592709856584No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT1950.2616080172795449No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC1530.20526167509625834No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA1220.16367270824668964Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC1190.15964796951931204Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGGC150.002218157869.9885942
CCTCTGA150.002218157869.98859465
GTCCTAA200.00691274552.526691
CGCATCT200.006931140652.4914424
CTCTGAC200.006931140652.4914466
AACACGA200.006931140652.4914411
TTTCGGA501.6901371E-435.01781
TTCGGAA604.944934E-429.1619132
TCTGCCA500.007173550327.99543427
ATGCAGA500.007173550327.99543410
TCTCGCC750.001818788623.3295298
AGCACCC800.002646535621.8714335
GACACTC6900.021.30087770
TAATCTC1003.890497E-420.99657670
TAGACGT1205.6333818E-520.4133425
ATCTCGA1055.40689E-419.9967387
GTCTGGG900.00523652719.4412752
GCACCCC900.00523652719.4412756
ATCTGAC900.00523652719.4412757
GGGACTG1107.395877E-419.08779735