Basic Statistics
Measure | Value |
---|---|
Filename | H3W35AFXY_n01_adr3wtzt14s7.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 74539 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 55 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC | 241 | 0.3233206777660017 | No Hit |
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC | 226 | 0.30319698412911367 | No Hit |
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC | 219 | 0.29380592709856584 | No Hit |
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT | 195 | 0.2616080172795449 | No Hit |
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC | 153 | 0.20526167509625834 | No Hit |
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA | 122 | 0.16367270824668964 | Illumina Multiplexing PCR Primer 2.01 (95% over 21bp) |
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC | 119 | 0.15964796951931204 | Illumina Multiplexing PCR Primer 2.01 (95% over 22bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TTTAGGC | 15 | 0.0022181578 | 69.988594 | 2 |
CCTCTGA | 15 | 0.0022181578 | 69.988594 | 65 |
GTCCTAA | 20 | 0.006912745 | 52.52669 | 1 |
CGCATCT | 20 | 0.0069311406 | 52.49144 | 24 |
CTCTGAC | 20 | 0.0069311406 | 52.49144 | 66 |
AACACGA | 20 | 0.0069311406 | 52.49144 | 11 |
TTTCGGA | 50 | 1.6901371E-4 | 35.0178 | 1 |
TTCGGAA | 60 | 4.944934E-4 | 29.161913 | 2 |
TCTGCCA | 50 | 0.0071735503 | 27.995434 | 27 |
ATGCAGA | 50 | 0.0071735503 | 27.995434 | 10 |
TCTCGCC | 75 | 0.0018187886 | 23.329529 | 8 |
AGCACCC | 80 | 0.0026465356 | 21.871433 | 5 |
GACACTC | 690 | 0.0 | 21.300877 | 70 |
TAATCTC | 100 | 3.890497E-4 | 20.996576 | 70 |
TAGACGT | 120 | 5.6333818E-5 | 20.41334 | 25 |
ATCTCGA | 105 | 5.40689E-4 | 19.996738 | 7 |
GTCTGGG | 90 | 0.005236527 | 19.441275 | 2 |
GCACCCC | 90 | 0.005236527 | 19.441275 | 6 |
ATCTGAC | 90 | 0.005236527 | 19.441275 | 7 |
GGGACTG | 110 | 7.395877E-4 | 19.087797 | 35 |