FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s78.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s78.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences216409
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT3670.16958629262184105No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC2940.13585386929379092No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC2540.11737034966198263No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC2530.11690826167118741No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT2330.10766650185528327No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA2330.10766650185528327No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT2190.10119726998415038No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGT207.914104E-570.000014
TCTAGGC400.002415110635.0000048
TTATAGT400.002415110635.0000044
GTTCTTA652.0961863E-532.30769340
CTTGTTA901.7475395E-731.11110957
GACGTAT450.004300812331.11110928
ACGTATA450.004300812331.11110929
GAGGATC500.00719764527.99999848
GTCTAGG500.00719764527.9999981
GTGCGAT500.00719764527.99999811
TTGACCA657.9383637E-426.9230774
AGTTAAT657.9383637E-426.9230772
ACCGTTA657.9383637E-426.9230778
CGCGTAA657.9383637E-426.92307721
GGATAAT1606.366463E-1126.2500021
ACTTGTG700.001223338925.050
ACACAGC700.001223338925.01
ACCTCTG1401.1796146E-825.016
AGTTCTT700.001223338925.039
TCTGTTC1701.382432E-1024.70588319
Produced by FastQC (version 0.11.2)