FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s77.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s77.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121013
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT3670.30327320205267205No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC2910.24047003214530668No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG2590.21402659218431075No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA2550.2107211621891863No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC2420.1999785147050317No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT2350.19419401221356383No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA2310.19088858221843935No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT2230.18427772222819036No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC2170.17931957723550362No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC2110.1743614322428169No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC1940.1603133547635378No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG1820.15039706477816434No Hit
GTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAA1700.14048077479279086No Hit
CATATAAGAACTCCACCGGTAATACGCTTACATACATAAAGGTATAGTAC1580.1305644848074174No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAG1520.12560633981473066No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC1520.12560633981473066No Hit
CATCTAAGGAAGGCAGCAGGCGCGTAAATTACCCACTCCCAGCTCGGGGA1490.12312726731838729No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT1390.11486369233057606No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA1380.11403733483179493No Hit
CCTTATGGGACATGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGAT1350.11155826233545157No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA1350.11155826233545157No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT1310.10825283234032707No Hit
GCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATGAA1260.10412104484642147No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT1260.10412104484642147No Hit
TTCTAGAGCTAATACATGCAATTAAAACATGAACCTTATGGGACATGTGC1240.10246832984885922No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGT150.002220083370.000015
TCTTAGC252.3824676E-455.9999962
GGGCTAT200.006937089452.51
GGATGTG200.006937089452.51
ATCCCCA200.006937089452.569
GACGAGT200.006937089452.568
GACGTAA352.0763142E-550.01
GTAATCA352.0763142E-550.04
TATAGCT305.858457E-446.66666866
GAGTTAA404.5703564E-543.7515
TTTCGGA350.001251321940.01
AAAAAAG350.001251321940.065
CTTGAAA350.001251321940.057
CTTAGCC350.001251321940.03
CGAGTTA459.153178E-538.88888514
ATCAATG459.153178E-538.8888857
AATGCGA459.153178E-538.88888510
TGCGAGT459.153178E-538.88888512
AGTTAAT459.153178E-538.88888516
CAATGCG459.153178E-538.8888859
Produced by FastQC (version 0.11.2)