FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s76.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s76.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences205181
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT4630.22565442219308804No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT3140.1530356124592433No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT2960.1442628703437453No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA2860.13938912472402415No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA2750.1340280045423309No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT2690.13110375717049824No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC2680.13061638260852612No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA2620.12769213523669345No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC2570.1252552624268329No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG2520.12281838961697233No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT2500.1218436404930281No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT2350.11453302206344643No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAG2240.10917190188175319No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGAGT150.002223244269.992454
CTCGTAT800.052.5071339
TAACGTG200.00694690452.494335
CCGTCTT850.049.40643347
GTAGGCG305.871176E-446.6616333
GTATGCC950.044.2165342
TCTCGTA950.044.2165338
GCCGTCT950.044.20575346
TATGCCG1050.040.00543243
TATCTCG1050.040.00543236
CTAGCCG350.00125252240.00543242
TAGAGTC350.001254024839.995684
CTGTAAG459.181327E-538.884691
TGCTTGA1100.038.18700455
GAGTAAC400.002413209335.00475365
AAAGGGG1201.8189894E-1235.00475368
ATGCCGT1201.8189894E-1235.00475344
ATTTTGA400.002416097834.99622322
CCGATCT13050.033.7894554
CTTCCGA13550.033.575711
Produced by FastQC (version 0.11.2)