Basic Statistics
Measure | Value |
---|---|
Filename | H3W35AFXY_n01_adr3wtzt14s75.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 225432 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 53 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT | 539 | 0.23909649029419072 | No Hit |
TCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT | 392 | 0.17388835657759324 | No Hit |
ATCACAAACATTTCAACTTCGCTGAGTCTCGGGAGGAGACAGTGTGGCCA | 290 | 0.1286418964477093 | No Hit |
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC | 282 | 0.125093154476738 | No Hit |
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC | 279 | 0.12376237623762376 | No Hit |
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC | 264 | 0.11710848504205258 | No Hit |
GTGCTGGAAGATTAATTGATGGGGTGCAAGCTCTTGATCGAAGTCCCAGT | 245 | 0.10868022286099578 | No Hit |
GGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTA | 239 | 0.1060186663827673 | No Hit |
GTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGG | 231 | 0.10246992441179602 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TCTATTG | 20 | 7.914819E-5 | 70.0 | 8 |
TCGCAAA | 20 | 0.006944825 | 52.5 | 8 |
GGCCTTA | 20 | 0.006944825 | 52.5 | 7 |
GTATTTG | 30 | 5.869294E-4 | 46.666668 | 1 |
TCTAGTA | 30 | 5.869294E-4 | 46.666668 | 8 |
CGTAATC | 35 | 0.0012536227 | 40.0 | 20 |
TCTAGGG | 50 | 1.7061329E-4 | 35.0 | 3 |
TCTGTCA | 40 | 0.0024153234 | 35.0 | 8 |
CTCTGCG | 40 | 0.0024153234 | 35.0 | 9 |
TATCTCG | 140 | 0.0 | 32.5 | 70 |
TAATGCC | 65 | 2.0965708E-5 | 32.307693 | 44 |
TCGTAAT | 55 | 2.9858807E-4 | 31.81818 | 35 |
AGTGAGT | 45 | 0.004301187 | 31.111113 | 2 |
TAATACT | 60 | 4.9717404E-4 | 29.166668 | 38 |
ATCTAGT | 60 | 4.9717404E-4 | 29.166668 | 7 |
CCGATTG | 60 | 4.9717404E-4 | 29.166668 | 9 |
GGTCCAA | 60 | 4.9717404E-4 | 29.166668 | 11 |
ACGCGGG | 60 | 4.9717404E-4 | 29.166668 | 12 |
CTTAATA | 75 | 5.568451E-5 | 28.000002 | 38 |
TTGGACT | 50 | 0.0071982704 | 27.999998 | 4 |