FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s75.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s75.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences225432
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT5390.23909649029419072No Hit
TCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT3920.17388835657759324No Hit
ATCACAAACATTTCAACTTCGCTGAGTCTCGGGAGGAGACAGTGTGGCCA2900.1286418964477093No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC2820.125093154476738No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC2790.12376237623762376No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC2640.11710848504205258No Hit
GTGCTGGAAGATTAATTGATGGGGTGCAAGCTCTTGATCGAAGTCCCAGT2450.10868022286099578No Hit
GGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTA2390.1060186663827673No Hit
GTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGG2310.10246992441179602No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATTG207.914819E-570.08
TCGCAAA200.00694482552.58
GGCCTTA200.00694482552.57
GTATTTG305.869294E-446.6666681
TCTAGTA305.869294E-446.6666688
CGTAATC350.001253622740.020
TCTAGGG501.7061329E-435.03
TCTGTCA400.002415323435.08
CTCTGCG400.002415323435.09
TATCTCG1400.032.570
TAATGCC652.0965708E-532.30769344
TCGTAAT552.9858807E-431.8181835
AGTGAGT450.00430118731.1111132
TAATACT604.9717404E-429.16666838
ATCTAGT604.9717404E-429.1666687
CCGATTG604.9717404E-429.1666689
GGTCCAA604.9717404E-429.16666811
ACGCGGG604.9717404E-429.16666812
CTTAATA755.568451E-528.00000238
TTGGACT500.007198270427.9999984