FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s74.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s74.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114837
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC1920.1671935003526738No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT1910.16632270087167028No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC1870.16283950294765623No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC1780.15500230761862466No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC1530.13323232059353693No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA1320.11494553149246324No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG1290.11233313304945271No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA1260.10972073460644217No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT1200.10449593772042112No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC1190.10362513823941762Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGTCG200.00693618852.54
CACCCGA200.00693618852.561
ATATAAG305.857196E-446.6666642
CTTGTCT501.7009291E-435.09
CTCGTAT601.2070521E-535.039
CATTGGG400.002410404235.02
TTGTCTC501.7009291E-435.010
CTGAACA501.7009291E-435.04
GTATGCC601.2070521E-535.042
GTCTTCT601.2070521E-535.049
TTATATG652.0876694E-532.3076932
GCCGTCT652.0876694E-532.30769346
GTCTCAA552.976818E-431.81818412
TATATGC552.976818E-431.8181843
TATGCTT552.976818E-431.8181845
CATATAA450.0042924831.1111131
TGCTTGA703.4638302E-530.00000255
GCTTGAA703.4638302E-530.00000256
AAAGGGG703.4638302E-530.00000268
ATATGCT604.95673E-429.1666664
Produced by FastQC (version 0.11.2)