FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s73.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s73.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157616
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT2810.17828139275200486No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT2590.16432341894223937No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG2390.15163435184245255No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA2330.1478276317125165No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC2330.1478276317125165No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC2190.13894528474266574No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA2110.133869657902751No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC2010.1275251243528576No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT1890.11991168409298548No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC1870.1186427773830068No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT1860.11800832402801746No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC1640.10405035021825194No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTGA200.006940968352.4999961
GCATTGG200.006940968352.4999961
CTTCGCT350.001252475340.09
GGTCCAA350.001252475340.011
CTCGTAT459.165803E-538.8888939
TCTTCTG459.165803E-538.8888950
TATGCCG459.165803E-538.8888943
GTATGCC459.165803E-538.8888942
TTTCGGA852.5502231E-937.0588231
CCGTCTT501.7038075E-435.00000447
TTCGGAA904.458343E-935.0000042
GTCTTCT501.7038075E-435.00000449
GCCGTCT501.7038075E-435.00000446
ATAGCGT450.004297297431.11111353
TAGATAC901.7424281E-731.1111135
CAAGAAT450.004297297431.11111315
AAGGACA450.004297297431.11111345
GCCGGGA703.4719644E-530.013
AAAAAGG703.4719644E-530.066
TATCTCG1103.1488526E-828.63636270
Produced by FastQC (version 0.11.2)