FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s72.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s72.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences354754
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC4430.12487526567706073No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA4330.12205641092137087No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT4250.11980132711681898No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAG4160.11726435783669811No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT3930.11078099189861144No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC3930.11078099189861144No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT3650.10288819858267984No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCTAG200.006949090452.498165
GATATAT400.002415944835.0036661
ATCTGAC1400.032.498827
TTTCGGA2050.032.442421
CGATCTG14950.032.306525
CCGATCT33550.031.817034
TCCGATC34150.031.5654743
TTCCGAT34250.031.3711262
GTCTACA450.00430227931.1143681
CTTCCGA34650.030.8113061
GTATACA604.978487E-429.165619
ATAGACA500.007205032327.9989854
ATCTGGG3600.027.2212377
GATCTGG7400.026.958486
ATCTAGC1056.756618E-726.66569912
TTCGGAA2500.026.5990352
TCTTACA1101.0139083E-625.4536232
GTACTTC1101.0139083E-625.4536236
CGATCTA8600.025.2316445
ATCTAGT700.001225141924.9990947
Produced by FastQC (version 0.11.2)