FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s71.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s71.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172700
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT4510.2611464968152866No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT4060.2350897510133179No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA3050.1766068326577881No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA2880.1667631731325999No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG2780.16097278517660682No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT2480.14360162130862766No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC2380.13781123335263462No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT2270.13144180660104227No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG2240.12970469021424436No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA2170.12565141864504922No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAG2160.1250723798494499No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC2010.11638679791546033No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT1980.11464968152866241No Hit
CATATAAGAACTCCACCGGTAATACGCTTACATACATAAAGGTATAGTAC1840.10654313839027214No Hit
CATCTAAGGAAGGCAGCAGGCGCGTAAATTACCCACTCCCAGCTCGGGGA1830.10596409959467284No Hit
CCTTATGGGACATGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGAT1810.10480602200347423No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT1770.10248986682107701No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCAG200.006943678552.4969610
AATCGTG200.006943678552.4969660
TCTAGGC459.172584E-538.886648
ATTAGCC400.002414450334.9979743
TTTCGGA904.465619E-934.9979741
GACGTAA400.002414450334.99797418
TCTTTTT2300.033.4763228
GCGTAAA652.0904303E-532.31517822
TAAGGTA652.094577E-532.3058245
ATCTGGG1001.2480086E-831.4981757
CTTTTTT2450.031.426759
CGCATGA450.00429964631.1093142
CGTCTTC901.7449565E-731.1093148
TCTAGTG450.00429964631.1093131
ATCTTTT2650.030.3755997
ATGTCTA1352.5465852E-1028.51686533
TTGTCTC1502.910383E-1127.99837910
TATGCTT1502.910383E-1127.9983795
GGACGTA500.00719571527.99837917
CACTTAA500.00719571527.99837964