FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s70.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s70.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158691
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT3320.20921161250480494No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC2540.16005948667536282No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC2240.14115482289480816No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC2240.14115482289480816No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC1670.1052359617117543No Hit
GACGTAATCAATGCGAGTTAATGACTCACACTTACTGGGAATTCCAAGTT1620.10208518441499519No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTATA252.385534E-455.9962229
GTCTATA200.00693425352.5130041
TCTGAGG200.00694290652.4964528
TGTATAG200.00694290652.4964522
TAACACG200.00694290652.49645270
TACTGAA200.00694290652.4964525
TATAGGT200.00694290652.4964524
TGTGTAT200.00694290652.4964523
GGGGATC305.8659614E-446.66351315
CGTATAG400.002410241935.0086730
ATAGGGT400.002410241935.0086733
TATAGGG400.002410241935.0086732
ACTCTGC400.002413973734.9976358
CTCGTAT652.0936466E-532.30550839
GTATGCC652.0936466E-532.30550842
GACGTAT450.004292174231.11881628
AGTGGAC450.004292174231.11881624
TGCAAAC450.00429880431.1090112
GTATAGG500.00718324528.00693731
GTAAACC755.548825E-528.00693522