Basic Statistics
Measure | Value |
---|---|
Filename | H3W35AFXY_n01_adr3wtzt14s70.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 158691 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 54 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT | 332 | 0.20921161250480494 | No Hit |
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC | 254 | 0.16005948667536282 | No Hit |
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC | 224 | 0.14115482289480816 | No Hit |
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC | 224 | 0.14115482289480816 | No Hit |
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC | 167 | 0.1052359617117543 | No Hit |
GACGTAATCAATGCGAGTTAATGACTCACACTTACTGGGAATTCCAAGTT | 162 | 0.10208518441499519 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
ACGTATA | 25 | 2.385534E-4 | 55.99622 | 29 |
GTCTATA | 20 | 0.006934253 | 52.513004 | 1 |
TCTGAGG | 20 | 0.006942906 | 52.496452 | 8 |
TGTATAG | 20 | 0.006942906 | 52.496452 | 2 |
TAACACG | 20 | 0.006942906 | 52.496452 | 70 |
TACTGAA | 20 | 0.006942906 | 52.496452 | 5 |
TATAGGT | 20 | 0.006942906 | 52.496452 | 4 |
TGTGTAT | 20 | 0.006942906 | 52.496452 | 3 |
GGGGATC | 30 | 5.8659614E-4 | 46.663513 | 15 |
CGTATAG | 40 | 0.0024102419 | 35.00867 | 30 |
ATAGGGT | 40 | 0.0024102419 | 35.00867 | 33 |
TATAGGG | 40 | 0.0024102419 | 35.00867 | 32 |
ACTCTGC | 40 | 0.0024139737 | 34.997635 | 8 |
CTCGTAT | 65 | 2.0936466E-5 | 32.305508 | 39 |
GTATGCC | 65 | 2.0936466E-5 | 32.305508 | 42 |
GACGTAT | 45 | 0.0042921742 | 31.118816 | 28 |
AGTGGAC | 45 | 0.0042921742 | 31.118816 | 24 |
TGCAAAC | 45 | 0.004298804 | 31.10901 | 12 |
GTATAGG | 50 | 0.007183245 | 28.006937 | 31 |
GTAAACC | 75 | 5.548825E-5 | 28.006935 | 22 |