H3W35AFXY_n01_adr3wtzt14s6.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H3W35AFXY_n01_adr3wtzt14s6.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	226985
Sequences flagged as poor quality	0
Sequence length	76
%GC	54

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC	1107	0.487697424939974	No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC	1045	0.4603828446813666	No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC	964	0.42469766724673436	No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC	779	0.34319448421701876	No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC	449	0.19781042800185034	Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA	412	0.18150979139590723	Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT	264	0.11630724497213472	No Hit
GAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTC	258	0.1136638984954953	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGACCG	35	2.0821339E-5	50.0	10
ATATAAG	45	1.6797749E-6	46.666664	2
ACAATGT	35	0.001253641	40.0	30
TAGGGGT	35	0.001253641	40.0	4
ATAAGAA	45	9.178577E-5	38.888885	4
CATATAA	55	6.6754837E-6	38.18182	1
TATAAGA	55	6.6754837E-6	38.18182	3
GGTAATA	50	1.70617E-4	35.0	18
ATGCTTG	60	1.2122575E-5	35.0	6
AAGATTA	50	1.70617E-4	35.0	18
TTTCGGA	55	2.985944E-4	31.818184	1
TATGCTT	55	2.985944E-4	31.818184	5
AATACGC	55	2.985944E-4	31.818184	21
CGCTTAC	55	2.985944E-4	31.818184	25
CTAGACC	45	0.00430125	31.111109	9
TGTCAAG	45	0.00430125	31.111109	29
AAAGGGG	45	0.00430125	31.111109	68
AGCACCC	140	1.2732926E-11	30.0	5
AATCTCG	175	0.0	30.0	70
AAAGATT	70	3.4786415E-5	30.0	17