FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s69.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s69.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146673
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC4100.2795333837857002No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC3690.25158004540713014No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC3370.22976280569702673No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC2870.19567336864999013No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT1850.12613091707403543No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG1760.11999481840556886No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC1580.10772262106863567Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA1530.10431367736393202No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAAAG150.002221368769.9972763
TTATAGT252.3846001E-455.99781813
TATTCGA200.006931720352.5158620
AGTTACC200.006931720352.5158617
AAAAGTC200.006941078752.49795565
ATTATTC200.006941078752.4979553
ATACGAC200.006941078752.4979553
GCTAATG200.006941078752.49795513
CGTTTAC352.0789725E-549.9980529
GTTTACT305.8636745E-446.6648530
TATAGTT305.8636745E-446.6648514
GTTATAG305.8636745E-446.6648512
ATAGTTA350.001250330740.0120815
CCGTTTA459.164792E-538.8873728
TTACTGG654.8330367E-737.6908432
TAGTTAC400.002409005835.01057416
ACCGTCC400.002413041434.998648
CTAGGAC501.703621E-434.998643
CTGCAAA652.0921627E-532.30643511
GACGCCT552.9815087E-431.8169442