Basic Statistics
Measure | Value |
---|---|
Filename | H3W35AFXY_n01_adr3wtzt14s69.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 146673 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 54 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC | 410 | 0.2795333837857002 | No Hit |
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC | 369 | 0.25158004540713014 | No Hit |
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC | 337 | 0.22976280569702673 | No Hit |
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC | 287 | 0.19567336864999013 | No Hit |
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT | 185 | 0.12613091707403543 | No Hit |
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG | 176 | 0.11999481840556886 | No Hit |
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC | 158 | 0.10772262106863567 | Illumina Multiplexing PCR Primer 2.01 (95% over 22bp) |
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA | 153 | 0.10431367736393202 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GTAAAAG | 15 | 0.0022213687 | 69.99727 | 63 |
TTATAGT | 25 | 2.3846001E-4 | 55.997818 | 13 |
TATTCGA | 20 | 0.0069317203 | 52.51586 | 20 |
AGTTACC | 20 | 0.0069317203 | 52.51586 | 17 |
AAAAGTC | 20 | 0.0069410787 | 52.497955 | 65 |
ATTATTC | 20 | 0.0069410787 | 52.497955 | 3 |
ATACGAC | 20 | 0.0069410787 | 52.497955 | 3 |
GCTAATG | 20 | 0.0069410787 | 52.497955 | 13 |
CGTTTAC | 35 | 2.0789725E-5 | 49.99805 | 29 |
GTTTACT | 30 | 5.8636745E-4 | 46.66485 | 30 |
TATAGTT | 30 | 5.8636745E-4 | 46.66485 | 14 |
GTTATAG | 30 | 5.8636745E-4 | 46.66485 | 12 |
ATAGTTA | 35 | 0.0012503307 | 40.01208 | 15 |
CCGTTTA | 45 | 9.164792E-5 | 38.88737 | 28 |
TTACTGG | 65 | 4.8330367E-7 | 37.69084 | 32 |
TAGTTAC | 40 | 0.0024090058 | 35.010574 | 16 |
ACCGTCC | 40 | 0.0024130414 | 34.99864 | 8 |
CTAGGAC | 50 | 1.703621E-4 | 34.99864 | 3 |
CTGCAAA | 65 | 2.0921627E-5 | 32.306435 | 11 |
GACGCCT | 55 | 2.9815087E-4 | 31.81694 | 42 |