Basic Statistics
Measure | Value |
---|---|
Filename | H3W35AFXY_n01_adr3wtzt14s68.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 289298 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 55 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT | 760 | 0.2627048925329591 | No Hit |
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC | 464 | 0.16038825017801714 | No Hit |
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC | 419 | 0.14483335522540772 | No Hit |
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC | 400 | 0.13826573291208374 | No Hit |
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT | 304 | 0.10508195701318364 | No Hit |
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC | 302 | 0.10439062834862321 | No Hit |
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA | 299 | 0.1033536353517826 | Illumina Multiplexing PCR Primer 2.01 (95% over 21bp) |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 296 | 0.10231664235494195 | No Hit |
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC | 295 | 0.10197097802266177 | Illumina Multiplexing PCR Primer 2.01 (95% over 22bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
ATAGTAG | 20 | 0.006945717 | 52.50208 | 3 |
TAATCTC | 30 | 5.8659207E-4 | 46.676582 | 17 |
CGCTTTT | 55 | 6.672104E-6 | 38.18993 | 26 |
CCTAGAC | 65 | 4.8518086E-7 | 37.693794 | 3 |
TCTAATC | 40 | 0.0024159818 | 35.001385 | 2 |
CGACTAA | 60 | 1.213078E-5 | 35.001385 | 51 |
TTAACGT | 50 | 1.7069292E-4 | 35.00138 | 69 |
CTAGACC | 85 | 1.0599615E-7 | 32.94248 | 4 |
TAACCCT | 55 | 2.9902975E-4 | 31.81394 | 55 |
GTCTTAA | 45 | 0.0042987117 | 31.11772 | 27 |
GTACCCT | 45 | 0.0043023513 | 31.112339 | 6 |
AGACGTA | 45 | 0.0043023513 | 31.112339 | 19 |
GTCCACA | 45 | 0.0043023513 | 31.112339 | 1 |
GGATTAT | 45 | 0.0043023513 | 31.112339 | 1 |
TAGACTG | 45 | 0.0043059937 | 31.106964 | 42 |
GTAAACC | 60 | 4.968998E-4 | 29.172863 | 22 |
GGTAAAC | 60 | 4.974032E-4 | 29.16782 | 21 |
TTCCTAG | 60 | 4.974032E-4 | 29.16782 | 1 |
GCGAGTC | 60 | 4.974032E-4 | 29.16782 | 23 |
TAGACCG | 85 | 3.9443257E-6 | 28.824669 | 5 |