FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s68.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s68.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences289298
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT7600.2627048925329591No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC4640.16038825017801714No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC4190.14483335522540772No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC4000.13826573291208374No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT3040.10508195701318364No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC3020.10439062834862321No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA2990.1033536353517826Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2960.10231664235494195No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC2950.10197097802266177Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGTAG200.00694571752.502083
TAATCTC305.8659207E-446.67658217
CGCTTTT556.672104E-638.1899326
CCTAGAC654.8518086E-737.6937943
TCTAATC400.002415981835.0013852
CGACTAA601.213078E-535.00138551
TTAACGT501.7069292E-435.0013869
CTAGACC851.0599615E-732.942484
TAACCCT552.9902975E-431.8139455
GTCTTAA450.004298711731.1177227
GTACCCT450.004302351331.1123396
AGACGTA450.004302351331.11233919
GTCCACA450.004302351331.1123391
GGATTAT450.004302351331.1123391
TAGACTG450.004305993731.10696442
GTAAACC604.968998E-429.17286322
GGTAAAC604.974032E-429.1678221
TTCCTAG604.974032E-429.167821
GCGAGTC604.974032E-429.1678223
TAGACCG853.9443257E-628.8246695