FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s67.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s67.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences234777
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT3440.14652201876674462No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC3440.14652201876674462No Hit
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA3000.12778083031983542No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC2900.12352146930917424No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC2900.12352146930917424No Hit
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCT2520.10733589746866176No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC2370.10094685595266999Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAAA252.3846296E-456.0117571
TTATAGT252.3871417E-455.9998324
TATAAAG350.001253747839.9998782
ACTATAC501.7063784E-434.9998973
CTAGTCG400.002415563234.99989342
TACTATA400.002415563234.9998932
GCACAAT450.004297129331.1176431
CTCGTAT450.004301612731.11101536
CCGTCTT450.004301612731.11101544
ATAGTTA450.004301612731.1110156
ATCTGTA450.004301612731.11101526
GAACCCT906.1483206E-627.2221444
CTCTCCG906.1483206E-627.2221432
TTAGAGG808.6420434E-526.2499263
GTTAGAG808.6420434E-526.2499262
GGACAGG808.6420434E-526.249926
AACAGAT959.356554E-625.7893967
TAGGACT1508.731149E-1025.666594
AGGGTTC1251.09246685E-725.19992440
TCAGGGT1251.09246685E-725.19992438
Produced by FastQC (version 0.11.2)