FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s66.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s66.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94145
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT1560.16570184290190662No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC1320.1402092516862287No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT1200.12746295607838973No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC1070.11365446916989749No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC1020.10834351266663127No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG980.10409474746401826No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTA207.89091E-570.015
AGACACC150.002218544570.048
TAGCCCT200.00693231852.55
CGCTCTA200.00693231852.57
TACTGTG200.00693231852.57
GGACGGT200.00693231852.56
TCACGTC200.00693231852.547
CATATAA305.8517756E-446.6666641
CCGGTAA305.8517756E-446.66666416
CGGTAAT305.8517756E-446.66666417
TACGCTT350.001249903240.00000423
ATATAAG350.001249903240.0000042
AGACCAT350.001249903240.0000046
TCCACCG350.001249903240.00000412
ACGCTTA350.001249903240.00000424
AACTCCA350.001249903240.0000049
GCATGTT350.001249903240.00000420
TATAAGA350.001249903240.0000043
GGTAATA400.002408201335.018
GTACAAG400.002408201335.01